REGIONAL CEREBRAL CIRCULATION AND METABOLISM

区域脑循环和代谢

• 批准号: 3781335
• 负责人: L SOKOLOFF
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3781335
• 关键词: antipyrine; autogenic training; autoradiography; bioenergetics; brain disorder diagnosis; brain metabolism; carbon; deoxyglucose; diagnosis design /evaluation; fluorine; glucose metabolism; hibernation; neurophysiology; nitric oxide; physiologic stressor; positron emission tomography; radionuclide diagnosis; radionuclides; squirrel

This project continues to be directed toward the development, validation, and application of methods for,measurement of physiological and biochemical processes in functional and structural regions of the nervous system in animals and man. The methods are first developed for use in animals with quantitative autoradiography and then adapted for use in man with PET. The [14C]iodoantipyrine ([14C]IAP) method for measuring local cerebral blood flow (ICBF) and the [14C]deoxyglucose ([14C]DG) method for measuring local cerebral glucose utilization (ICMRgtc) are examples of methods that were developed in this program; adaptations of these methods are currently the only generally accepted quantitative methods routinely used in man with PET. These methods are applied to various physiological, pharmacological, and pathological states. Some of the major efforts in this project during the past year were as follows: l) model development and evaluation to simplify and to improve the accuracy and reliability of the [18F]fluorodeoxyglucose ([18F]FDG) adaptation of the (14C]DG method for human use with PET; 2) studies of the effects of stress produced by interference of glucose metabolism on local blood flow in the brain; 3) effects of hibernation on local cerebral blood flow and glucose utilization in the hibernating ground squirrel; 4) studies of the neurogenic control of the cerebral circulation; 5) development of a method to facilitate the determination of needed parameters to adapt the deoxyglucose method for use in pathological tissues and pathophysiological conditions; 6) studies of the role of nitric oxide in the coupling of cerebral blood flow to local cerebral energy metabolism and/or functional activity (described separately in another project report, 1.e., #02590-03).

该项目继续致力于发展， 生理学测量方法的验证和应用 以及细胞功能和结构区域的生化过程 动物和人类的神经系统。这些方法首先是为 在动物中使用定量放射自显影，然后适用于 使用PET的人。 [14 C]碘安替比林（[14 C]IAP）法 测量局部脑血流量（ICBF）和[14 C]脱氧葡萄糖 用于测量局部脑葡萄糖利用的（[14 C]DG）方法 （ICMRgtc）是在该计划中开发的方法的示例; 这些方法的修改是目前唯一被普遍接受的 常规用于人体PET的定量方法。 这些方法 应用于各种生理、药理和病理 states. 在过去一年中，该项目的一些主要努力 模型开发和评估，以简化和 为了提高[18F]氟脱氧葡萄糖的准确性和可靠性， （[18 F]FDG）（14 C]DG方法的适应性，用于PET的人体使用; 2） 葡萄糖干扰应激效应的研究 新陈代谢对大脑局部血流的影响; 3）冬眠的影响 对冬眠期局部脑血流量和葡萄糖利用的影响 地松鼠; 4）大脑神经原性控制的研究 循环; 5）开发一种方法，以便于确定 所需的参数，以适应脱氧葡萄糖方法用于 病理组织和病理生理条件; 6）研究 一氧化氮在脑血流量与脑血流耦合中的作用 局部脑能量代谢和/或功能活动（描述 在另一个项目报告中，1.e.，#02590-03）。