REGIONAL CEREBRAL CIRCULATION AND METABOLISM

该项目旨在开发、验证和 生理生化测定方法的应用 神经系统的功能和结构区域中的过程。 该方法首先被设计用于具有定量分析的动物。 放射自显影，然后适用于人体PET。 的 一种测定局部脑血的[14 C]碘安替比林（[14 C]IAP）法 流量（1CBF）和[14 C]脱氧葡萄糖（[14 C]DG）方法进行测量 局部脑葡萄糖利用（1CMR-glc）在这一过程中被开发出来。 程序;这些方法的改编是唯一普遍接受的 常规用于人体PET的定量方法。 这些方法 应用于各种生理、药理和病理 states. 在过去的一年里，我们努力实现这些目标：1） 评价将DG方法应用于病理学的必要参数 这些参数的值可能偏离正常值时的状态; 2） 开发连续双标记程序以研究对照和 在同一只动物中改变行为状态， 方法; 3）模型开发和评估，以提高准确性， [18F]氟脱氧葡萄糖（[18F]FDG）适应性的可靠性 （14 C]DG方法用于人体PET。

This project is directed toward the development, validation, and application of methods for measurement of physiological and biochemical processes in functional and structural regions of the nervous system. The methods are first designed for use in animals with quantitative autoradiography and then adapted for use in man with PET. The [14C]iodoantipyrine ([14C]IAP) method for measuring local cerebral blood flow (1CBF) and the [14C]deoxyglucose ([14C]DG) method for measuring local cerebral glucose utilization (1CMR-glc) were developed in this program; adaptations of these methods are the only generally accepted quantitative methods routinely used in man with PET. These methods are applied to various physiological, pharmacological, and pathological states. Efforts this past year have been directed toward these goals: 1) evaluation of necessary parameters to apply the DG method to pathological states when the values of these parameters may deviate from normal; 2) development of a sequential double label procedure to study control and altered behavioral states in the same animal with the autoradiographic methods; 3) model development and evaluation to improve accuracy and reliability of the [18F]fluorodeoxyglucose ([18F]FDG) adaptation of the (14C]DG method for human use with PET.