REGIONAL CEREBRAL CIRCULATION AND METABOLISM

区域脑循环和代谢

• 批准号: 3944659
• 负责人: L SOKOLOFF
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3944659
• 关键词: aging; animal old age; autoradiography; bioenergetics; brain circulation; brain mapping; brain metabolism; deoxyglucose; diagnosis quality /standard; glucose metabolism; mature animal; morphine; neuroanatomy; newborn animals; positron emission tomography; radionuclide diagnosis; radiotracer

The deoxyglucose method for the quantitative determination of rates of local glucose consumption in the discrete functional and structural components of the brain of conscious or anesthetized laboratory animals was developed in this laboratory over 10 years ago. In this method (14C)deoxyglucose is employed as a tracer for glucose flux through the hexokinase step; the product, (14C)deoxyglucose-6-phosphate, is measured by quantitative autoradiography. The method continues to be used to study alterations in local energy metabolism in a variety of physiological, pharmacological and a limited number of pathological states. Its suitability to a wider range of pathologic conditions is being extended and special time constraints which may be present in the method's adaptation for use in human subjects with (18C)fluorodeoxyglucose and PET have been examined.

脱氧葡萄糖法定量测定血药浓度 局部葡萄糖消耗率在离散泛函和 清醒或麻醉状态下大脑的结构成分 这个实验室培育了10多只实验动物。 几年前。在这种方法(14C)中，脱氧葡萄糖被用作 己糖激酶步骤中葡萄糖通量的示踪剂；该产品， (14C)脱氧葡萄糖-6-磷酸，通过定量测定 放射自显影。这种方法将继续被用于研究 多种疾病患者局部能量代谢的改变 生理的，药理的和数量有限的 病态。它适用于更广泛的病理学 条件正在延长，特殊的时间限制 可能存在于该方法的改编中，用于人类 患有(18C)氟脱氧葡萄糖和PET的受试者已经接受了 检查过了。