REGIONAL CEREBRAL CIRCULATION AND METABOLISM

这个项目将继续向发展方向发展， 生理学测量方法的验证和应用 和生化过程中的功能和结构区域 动物和人类的神经系统。这些方法最初是为 在动物身上使用定量放射自显影，然后改装成 用于患有PET的男士。[14C]碘安替比林([14C]IAP)法 测定局部脑血流量(ICBF)和[14C]脱氧葡萄糖 测量局部脑葡萄糖利用的([14C]DG)方法 (ICMRgtc)是在该计划中开发的方法的例子； 这些方法的改编是目前唯一被普遍接受的方法 定量方法是常规用于男性正电子发射计算机断层扫描。这些方法是 适用于各种生理、药理、病理 各州。在过去的一年里，这个项目的一些主要工作 分别是：L)模型的开发和评估，简化和 提高[18F]氟代脱氧葡萄糖的准确度和可靠性 ([18F]FDG)将(14C]DG方法改编为人类使用的PET；2) 葡萄糖干扰应激效应的研究 代谢对脑局部血流的影响；3)冬眠的影响 冬眠状态下局部脑血流量和葡萄糖利用的研究 地松鼠；4)大脑神经源性控制的研究 流通领域；5)开发一种便于测定的方法 使脱氧葡萄糖方法适用于 病理组织和病理生理条件；6)研究 一氧化氮在脑血流偶联中的作用 局部大脑能量代谢和/或功能活动(描述 另一份项目报告，1.E.，#02590-03)。

This project continues to be directed toward the development, validation, and application of methods for,measurement of physiological and biochemical processes in functional and structural regions of the nervous system in animals and man. The methods are first developed for use in animals with quantitative autoradiography and then adapted for use in man with PET. The [14C]iodoantipyrine ([14C]IAP) method for measuring local cerebral blood flow (ICBF) and the [14C]deoxyglucose ([14C]DG) method for measuring local cerebral glucose utilization (ICMRgtc) are examples of methods that were developed in this program; adaptations of these methods are currently the only generally accepted quantitative methods routinely used in man with PET. These methods are applied to various physiological, pharmacological, and pathological states. Some of the major efforts in this project during the past year were as follows: l) model development and evaluation to simplify and to improve the accuracy and reliability of the [18F]fluorodeoxyglucose ([18F]FDG) adaptation of the (14C]DG method for human use with PET; 2) studies of the effects of stress produced by interference of glucose metabolism on local blood flow in the brain; 3) effects of hibernation on local cerebral blood flow and glucose utilization in the hibernating ground squirrel; 4) studies of the neurogenic control of the cerebral circulation; 5) development of a method to facilitate the determination of needed parameters to adapt the deoxyglucose method for use in pathological tissues and pathophysiological conditions; 6) studies of the role of nitric oxide in the coupling of cerebral blood flow to local cerebral energy metabolism and/or functional activity (described separately in another project report, 1.e., #02590-03).