EFFECTS OF CHRONIC BROMIDE INTOXICATION ON LOCAL CEREBRAL GLUCOSE UTILIZATION

慢性溴化物中毒对局部脑葡萄糖利用的影响

• 批准号: 6162943
• 负责人: L SOKOLOFF
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6162943
• 关键词: brain metabolism; bromine; chronic disease /disorder; deoxyglucose; glucose metabolism; halogen poisoning; laboratory rat; neurotoxicology

Chronic bromide intoxication can produce a schizophrenia-like syndrome in man. To determine which structures in brain might be involved, we are studying local cerebral glucose utilization (LCGU) with the [14C]deoxyglucose method in rats being administered bromides chronically in the drinking water for up to 10 days and in both the water and by osmotic pump for up to 49 days. In rats given bromides only in the drinking water serum bromide levels were raised to about 200 mg NaBr/l in 2 days, 400 mg/l in 6 days, and 425 mg/l in 10 days. In control rats given only water serum bromide levels were between 0 and 12.3 mg/l. In rats given bromides in both drinking water and by osmotic pump serum bromide levels averaged 420 mg/l by 14 days, 540 mg/l by 21 days, 540 mg/l by 35 days, and 525 mg/l by 49 days. In control rats serum bromide levels ranged between 0 and 59 mg/l. Average rates of glucose utilization in the brain as a whole have thus far been determined in 34 rats. In rats treated for 21 days with bromide rates of glucose utilization were decreased by 12.5% compared with controls (p<0.05), grouped t-test). Densitometric analyses and image-processing of the autoradiograms needed to obtain rates of glucose utilization in individual regions of the brain are very labor-intensive procedures, and because of the considerable loss of personnel that we suffered during the past two years, we have been compelled to suspend this project, temporarily we hope.

慢性溴化物中毒可导致精神分裂样综合征 在人类身上。为了确定可能涉及大脑中的哪些结构，我们正在 脑部局部葡萄糖利用的研究 [14C]溴化物慢性染毒大鼠的脱氧葡萄糖方法 在饮用水中最多10天，在水中和 渗透泵使用时间长达49天。在大鼠中只给予溴化物 饮用水血清溴水平提高至约200毫克溴化钠/L 2天400 mg/L，6天400 mg/L，10天425 mg/L。在对照组大鼠中 只给水，血清溴水平在0到12.3 mg/L之间。 饮用水和渗透压泵血清中同时给予溴化物的大鼠 14天前平均水平为420毫克/L，21天前为540毫克/L L剂量为35天，L剂量为525 mg/次，剂量49天。对照组大鼠血清中的溴化物 水平在0到59毫克/L之间。平均血糖速率 到目前为止，在整个大脑中的利用率已确定为34 老鼠。在用溴化葡萄糖治疗21天的大鼠中 利用率与对照组相比下降了12.5%(p&lt；0.05)， 分组t检验)。密度学分析与图像处理 获得葡萄糖利用率所需的放射自显影 大脑的个别区域是非常劳动密集型的程序， 因为我们遭受了相当大的人员损失 在过去的两年里，我们被迫暂停了这个项目， 暂时我们希望如此。