IMMUNORESPONSE TO THE PRE-S GENE PRODUCT OF HBV

对 HBV 前 S 基因产物的免疫反应

• 批准号: 3874306
• 负责人: J W SHIH
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874306
• 关键词: antibody specificity; antiviral antibody; hepatitis B; hepatitis B antigens; human tissue; immunogenetics; virus genetics; virus infection mechanism; western blottings

Sera of HBV infected patients, HBV vaccine recipients and HBsAg immunized animals have been examined for their specific reaction to the pre-S gene product. Purified HBsAg is used as the source of material and is subjected to SDS-PAGE electrophoresis and protein blot (Western blot). Polypeptides encoded by the pre-S gene are confirmed by reaction with specific antibodies and their binding to polymerized human albumin. The immunoresponse is studied by its reaction to the specific polypeptides in the protein blots and its serological profile.

乙型肝炎病毒感染者、乙肝疫苗接种者和HBsAg免疫者血清 动物对前S基因的特异性反应已经被检测过了 产品纯化的HBsAg用作材料来源，并进行 SDS-PAGE电泳和蛋白质印迹（Western blot）。多肽 由前S基因编码的蛋白质通过与特异性 抗体及其与聚合人白蛋白的结合。的 免疫应答通过其对特定多肽的反应来研究， 蛋白质印迹及其血清学特征。