CHARACTERIZATION OF HUMAN PATHOGENIC MYCOPLASMA FROM HIV INFECTED PATIENTS

HIV 感染者的人类致病性支原体的特征

• 批准号: 5201142
• 负责人: J W SHIH
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201142
• 关键词: HIV infections; Mycoplasma; antimicroorganism antibody; bacterial genetics; communicable disease diagnosis; genetic strain; microorganism classification; microorganism culture; nucleic acid probes; opportunistic infections; serology /serodiagnosis; solution hybridization; species difference; urinary tract disorder diagnosis; urinary tract infection

The laboratory continues to work on newly identified strains of mycoplasma originated from AIDS patients. One of the isolates: Mycoplasma penetrans, is a new species of mycoplasma isolated from the urogenital tract of patients with AIDS. Genetic analysis of the DNA of this agent by liquid hybridization procedure has shown that this agent is a new species. This organism has properties of adherence, hemadsorption, cytasorption, and invades many different types of mammalian cells. This, it was so named: M penetrans. Specific genetic clones and antibodies against this agent were prepared and used to probe infected tissues. Attempts have been made to develop molecular and serologic assays for the agent and to measure specific immunoresponses. These assays were used to assess the clinical significance and pathogenicity of this agent in humans. The development of species-specific serological tests for mycoplasmas was a unique accomplishment by our group. We continue to explore new tests for other mycoplasmas in addition to the existing ones for Mycoplasma fermentens, M. Penetrans, M genitilium, and M. Pneumonaie. We applied these serological tests to several clinical settings, including patients with HIV infection, non-gonococcal urethritis (UGE), sexually transmitted diseases, and intravenous drug use. We found high prevalences of antibodies to M. Penetrans in patients with Kaposi's sarcoma and antibodies to M. Genitalium in patients with NGU. Using paired donor- recipient specimens, we also found M. Fermentens and M. Genitalium were transmissible through blood transfusion.

该实验室继续研究新发现的支原体菌株。 起源于艾滋病患者。其中一个分离株：穿透支原体， 是一种新的支原体，分离自猪的泌尿生殖道。 艾滋病患者。该菌剂DNA的液体遗传分析 杂交试验表明，该菌剂为新种。这 生物体具有黏附、血吸附、细胞吸附和 入侵多种不同类型的哺乳动物细胞。这个，它就是这样命名的：M 穿透者。针对该病原体的特异性基因克隆和抗体 准备好并用于探测受感染的组织。已经有人尝试过 建立该试剂的分子和血清学分析方法，并测量 特异性免疫反应。这些检测被用来评估临床 这种病原体在人类中的意义和致病性。 支原体的物种特异性血清学试验的发展是 这是我们团队独一无二的成就。我们继续探索新的测试 除现有支原体外的其他支原体 Fermentens、M.Penetrans、M.genitilum和M.Penonaie。我们申请了 这些血清学测试适用于多个临床环境，包括患者 感染艾滋病毒，非淋菌性尿道炎(UGE)，性传播 疾病，以及静脉注射毒品。我们发现有很高的发病率 卡波西肉瘤患者血清中抗穿透支原体抗体的检测 非淋菌性尿道炎患者生殖道支原体抗体的检测使用配对捐赠者- 受者标本中，我们还发现发酵分枝杆菌和生殖分枝杆菌 可通过输血传播的。