CHARACTERIZATION OF HUMAN PATHOGENIC MYCOPLASMA FROM HIV INFECTED PATIENTS

HIV 感染者的人类致病性支原体的特征

• 批准号: 3752235
• 负责人: J W SHIH
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752235
• 关键词: HIV infections; Mycoplasma; antimicroorganism antibody; bacterial genetics; communicable disease diagnosis; genetic strain; microorganism classification; microorganism culture; molecular cloning; nucleic acid probes; opportunistic infections; solution hybridization; species difference; urinary tract disorder diagnosis; urinary tract infection

The laboratory continues to work on newly identified strains of mycoplasma originated from AIDS patients. One of the isolates: Mycoplasma penetrans, is a new species of mycoplasma isolated from the urogenital tract of patients with AIDS. Genetic analysis of the DNA of this agent by liquid hybridization procedure has shown that this agent is a new species. This organism has properties of adherence, hemadsorption, cytadsorption and invades many different types of mammalian cells. Thus, it was so named: M. penetrans. Specific genetic clones and antibodies against this agent were prepared and were used to probe infected tissues. Attempts have been made to develop molecular and serological assays for the agent and specific immunoresponse to this agent. These assays were used to assess the significance of infectivity and pathogenicity of this agent in humans. This project is part of the continued research and development effort of this laboratory to investigate the co-factors contributing to the pathogenesis of AIDS. Many of the tests and investigating procedures developed in this study were incorporated in the enhancement or direct service ability of this laboratory.

实验室继续研究新发现的 支原体来源于艾滋病患者。 分离株之一： 反式支原体（Mycoplasma acetyltransis）是一种从大肠杆菌中分离到的新的支原体 艾滋病患者的泌尿生殖道。 DNA的遗传分析 通过液体杂交程序对该试剂的研究表明， 代理人是一个新物种。 这种微生物具有粘附性， 红细胞吸附，细胞吸附和侵入许多不同类型的 哺乳动物细胞 因此，它被命名为：M。是的。 具体 制备针对该试剂的遗传克隆和抗体， 被用来探测受感染的组织 已经尝试 发展分子和血清学分析， 免疫反应。这些试验用于评估 该病原体在人类中的感染性和致病性的重要性。 该项目是持续研究和开发工作的一部分 本实验室调查的辅助因素有助于 艾滋病的发病机制。 许多测试和调查程序 在这项研究中开发的被纳入增强或直接 实验室的服务能力。