ANIMAL MODEL OF NEUROPSYCHIATRIC DISORDERS

神经精神疾病的动物模型

• 批准号: 3845218
• 负责人: J N CRAWLEY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3845218
• 关键词: brain metabolism; caudate nucleus; cholecystokinin; cocaine; disease /disorder model; dopamine receptor; enkephalins; in situ hybridization; infant animal; laboratory rat; membrane transport proteins; messenger RNA; method development; model design /development; nervous system disorder; nucleic acid probes; nucleus accumbens; substantia nigra; tegmentum; tyrosine 3 monooxygenase

In situ hybridization analysis of mRNAs for tyrosine hydroxylase, cholecystokinin, enkephalin, the D-1 and D-2 dopamine receptor subtypes, and the dopamine transporter, in brains from rats treated neonatally with cocaine, are almost completed. No difference between cocaine treatment and dietary controls has been seen in mRNA concentrations in the ventral tegmentum, substantia nigra, caudate nucleus, or nucleus accumbens, for TH, CCK, ENK, D-1, or D-2 mRNAs. Dr. de Bartolomeis has recently developed a new oligonucleotide probe for the dopamine transporter, which he has characterized for specificity, and is now quantitating in the prenatal cocaine experiment.

酪氨酸羟化酶mRNA的原位杂交分析， 胆囊收缩素，脑啡肽，D-1和D-2多巴胺受体亚型， 和多巴胺转运蛋白，在接受脑内注射 可卡因，几乎完成了。 可卡因治疗之间没有差异 在腹侧的mRNA浓度中观察到饮食控制 被盖、黑质、尾状核或延髓核，用于 TH、CCK、ENK、D-1或D-2 mRNA。 de Bartolomeis博士最近 开发了一种新的多巴胺转运蛋白寡核苷酸探针， 他已经确定了特异性的特征，现在正在 产前可卡因实验