BIOPHYSICAL INSTRUMENTATION AND METHODOLOGY

生物物理仪器和方法

• 批准号: 3896158
• 负责人: M S LEWIS
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3896158
• 关键词: analytical ultracentrifugation; biomedical equipment development; computer data analysis; computer simulation; macromolecule; mathematical model; model design /development; personal computers

The project is designed to develop new instrumentation and methods and to improve existing instrumentation and techniques for the characterization of biological macromolecules and for study of their interactions. Analytical ultracentrifugation, the techniques ancillary to it, and methods of data analysis using mathematical modeling appropriate for these techniques are the major areas of interest. Studies on the application of mathematical modeling to problems of ultracentrifugal analysis have continued. The applications of these studies are described in the project report 10184-07. Further progress has been made in the development of data acquisition systems. work is continuing on a direct digital acquisition system using the Macintosh II. New methods of performing experiments and analyzing data have been developed and work is continuing in this area. In particular, (1) extensive studies on the concentration dependence of sedimentation coefficients have been investigated; (2) the PC version of MLAB has been very extensively used and evaluated; (3) studies on the possible application of singular value decomposition techniques to the study of data from heterogeneous associations is being initiated.

该项目旨在开发新的仪器和方法， 改进现有的仪器和技术， 生物大分子及其相互作用的研究。分析 超浓缩技术及其辅助技术和数据处理方法 使用适合于这些技术的数学建模的分析是 感兴趣的主要领域。 数学建模在工程问题中的应用研究 超临界分析仍在继续。这些研究的应用 在项目报告10184-07中进行了描述。了新的进展 在数据采集系统的开发过程中做出的。工作仍在继续， 使用Macintosh II的直接数字采集系统。的新方法 进行实验和分析数据已经开发和工作是 继续在这个领域。特别是，（1）广泛研究 沉降系数的浓度依赖性已经被 调查;（2）MLAB的PC版本已被广泛使用， （3）奇异值的可能应用研究 分解技术来研究异构数据 协会正在启动。