BIOPHYSICAL INSTRUMENTATION AND METHODOLOGY

该项目旨在开发新的仪器和 用于表征的方法(或改进现有方法) 生物大分子及其相互作用的研究。 分析超速离心法及其辅助技术，以及 使用数学建模进行数据分析的方法 适合这些技术的是主要感兴趣的领域。 在本项目中，主要侧重于应用 超速离心分析中问题的数学建模。 这个令人关注的原则一直是扩展 对更广泛的大分子研究的隐性限制 相互作用，特别是分析组合的同质 和异质关联。MLab软件，在 DEC-10型计算机，已用于数学建模。 这些研究的应用在项目中进行了描述。 报告10184-04，生物物理化学 大分子。 继续进行可行性研究，以开发一种低成本的 光学数据采集系统(基于荧光扫描) 这将把现有的预备性超速离心机转化为 分析性超速离心机。

The project is designed to develop new instrumentation and methods (or to improve existing ones) for characterization of biological macromolecules and for study of their interactions. Analytical ultracentrifugation, the techniques ancillary to it, and methods of data analysis using mathematical modeling appropriate for these techniques are the major areas of interest. Major emphasis in this project has been given to applications of mathematical modeling to problems in ultracentrifugal analysis. This principal of concern has been to extend the method of implicit constraints to a wider range of studies of macromolecular interactions and in particular analyzing combined homogeneous and heterogeneous associations. MLAB software, operating on the DEC-10 computer, has been used for the mathematical modeling. The applications of these studies are described in the project report 10184-04, Physical Chemistry of Biological Macromolecules. A feasibility study continues toward development of a low-cost optical data acquisition system (based upon fluorescence scanning) that would convert existing preparative ultracentrifuges into analytical ultracentrifuges.