BIOPHYSICAL INSTRUMENTATION AND METHODOLOGY

生物物理仪器和方法

• 批准号: 3874157
• 负责人: M S LEWIS
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874157
• 关键词: analytical ultracentrifugation; biomedical equipment development; computer data analysis; glycoproteins; human tissue; mathematical model; model design /development; molecular weight; receptor; sedimentation; sedimentation velocity

The project is designed to develop new instrumentation and methods, and to improve existing instrumentation and techniques, for the characterization of biological macromolecules and the study of their interactions. Analytical ultracentrifugation, the techniques ancillary to it, and methods of data analysis using mathematical modeling appropriate for these techniques are the major areas of interest. Studies on the application of mathematical modeling to problems of ultracentrifugal analysis, and the development of new methods of performing experiments and analyzing data, have continued. In particular: (1) Extensive studies on the concentration dependence of sedimentation coefficients at very high protein concentrations have been continued. (2) Application of the method of implicit constraints to the analysis of simultaneous homogeneous and heterogeneous associations has been achieved. (3) A method has been developed for obtaining the upper and lower limits of the molecular weight of a glycoprotein when the composition of the carbohydrate portion is unknown. (4) A method has been developed for measuring the interaction of proteins with receptors having a heterogeneous molecular weight distribution. (5) A new method for the analysis of sedimentation velocity experiments has been developed.

该项目旨在开发新的仪器和方法，并 改进现有的仪器和技术， 生物大分子及其相互作用的研究。 分析超离心及其辅助技术和方法 数据分析使用数学建模适合这些 技术是感兴趣的主要领域。 数学建模在工程问题中的应用研究 超离心分析，以及新的表演方法的发展， 实验和分析数据，继续进行。 具体而言：（1） 关于沉降的浓度依赖性的广泛研究 在非常高的蛋白质浓度下的系数已经继续。（二） 隐式约束法在结构分析中的应用 实现了同时的同质和异质关联。 (3)本文提出了一种求取上下限的方法， 当糖蛋白的组成不同时， 碳水化合物部分是未知的。(4)已经开发了一种方法， 测量蛋白质与具有异质性的受体的相互作用 分子量分布(5)一种新的分析方法， 沉积速度实验已经开发。