CELLULAR BIOCHEMISTRY STUDIES ON CHEMICAL SELECTED FOR EVALUATION BY NTP

NTP 选定用于评估的化学物质的细胞生物化学研究

• 批准号: 3965209
• 负责人: M P DIETER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3965209
• 关键词: T lymphocyte; aging; animal old age; chemical carcinogenesis; disease /disorder model; environmental contamination; environmental toxicology; immunotoxicity; macrophage; mercury; mercury poisoning; metal complex; metal poisoning; nickel; organometallic compounds; oxygenases; renal toxin; reticuloendothelial system; titanium; unspecific monooxygenase

The effect of inorganic or organic metals and metal complexes is of particular interest to the NTP because of their prevalence in drinking water and industrial processes, use as constituents in anticancer drugs, and their diverse target organ toxicities. Mechanisms of cellular immunotoxicity were studied in rats or mice exposed to mercuric chloride, nickel sulfate or titanocene dichloride. Tissue accumulation of these metals in target organs indicated the sensitivity of the biochemical assays to evaluate target organ toxicity which often preceeded gross, microscopic or clinical methods. Development of animal models to study Fischer rat leukemia revealed tumor markers for this disease that can be use to distinguish between age-induced and chemically-enhanced leukemogenesis. Urinary enzyme responses to nephrotoxic chemical insult were evaluated to use as a model to predict renal toxicity in chronic studies. Enzymatic method development to enhance stability, sensitivity, and efficiency of 7-ethoxycoumarin-ortho-demethylase was initiated to evaluate MFO-initiating activity of chemicals in minute amounts of rodent tissue.

无机或有机金属和金属络合物的作用 NTP特别感兴趣，因为它们 在饮用水和工业生产过程中的普遍程度， 抗癌药物中的成分及其不同的靶点 器官毒性 在大鼠中研究了细胞免疫毒性机制， 暴露于氯化汞、硫酸镍或 二氯二茂钛。 这些金属的组织蓄积， 靶器官表明生化敏感性 用于评价靶器官毒性的测定， 肉眼、显微镜或临床方法。 发展畜牧 研究Fischer大鼠白血病的模型揭示了肿瘤标志物 可以用来区分 年龄诱导和化学增强的白血病。 尿 评价了对肾毒性化学损伤的酶反应 作为模型来预测慢性肾毒性 问题研究 开发酶促方法以提高稳定性， 灵敏度和效率 启动7-乙氧基香豆素-邻-脱甲基酶以评估 MFO-引发微量化学品的活性 啮齿动物组织