ACETYLATION PHENOTYPE OF ALCOHOLICS

酒精的乙酰化表型

• 批准号: 4687735
• 负责人: E A LANE
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4687735
• 关键词: acetylation; alcoholism /alcohol abuse; blood chemistry; drug metabolism; gene expression; high performance liquid chromatography; human subject; metabolism disorder; pharmacokinetics; urinalysis

Any coincidence of the slow acetylator phenotype and alcoholism will be investigated in this study. The proportion of alcoholic subjects that exhibits the slow acetylator phenotype is to be compared with the proportion of normal volunteers that exhibits the slow acetylator phenotype. The acetylator phenotype will be measured in normal volunteers and recovered alcoholics who are not related to each other. The recovered alcoholics should have a history of alcoholism, and the normal volunteers should be nonalcoholic and have no first-degree relative who is alcoholic. The acetylator phenotype will be determined after a single dose of sulfamethazine by measurement of blood and urine concentrations of sulfamethazine and its acetylated metabolite. These will be measured by high-pressure liquid chromatography. The acetylator phenotype has been determined in 15 alcoholics and 10 unrelated normal volunteers. The number of subjects is insufficient to draw any conclusions regarding the relative distribution of the slow acetylator phenotype.

慢乙酰化表型和酗酒的任何巧合都将是 在这项研究中调查。 酗酒者中表现出缓慢乙酰化的比例 将表型与正常志愿者的比例进行比较， 表现出慢乙酰化表型。 将在正常志愿者中测量乙酰化表型， 彼此没有血缘关系的戒酒者。 回收的 酗酒者应该有酗酒史，而正常的志愿者 不酗酒，没有酗酒的一级亲属。 乙酰化因子表型将在单次给药后确定。 磺胺二甲嘧啶血、尿浓度测定法 磺胺二甲嘧啶及其乙酰化代谢物。 这些将通过以下方式进行衡量： 高压液相色谱法 在15名酗酒者和10名 正常志愿者。 受试者数量不足， 得出任何关于缓慢的相对分布的结论 乙酰化表型