BIOCHEMICAL METHODS FOR VASOACTIVE SUBSTANCES

血管活性物质的生化方法

• 批准号: 4694553
• 负责人: D S GOLDSTEIN
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4694553
• 关键词: atrial natriuretic peptide; blood chemistry; catecholamines; cerebrospinal fluid; clinical chemistry; dihydroxyphenylalanine; electrochemistry; high performance liquid chromatography; human subject; hypertension; neuroblastoma; pheochromocytoma; plasma; radioimmunoassay; saluresis; urinalysis; vasoactive agent

We have established methodology for measuring plasma, urine, and cerebrospinal fluid levels of DOPA, DOPAC, and the catecholamine using liquid chromatography with electrochemical detection. One source of contaminating peaks in the assay is dihydrocaffeic acid, a metabolite of caffeic acid, which is a catechol found in coffee. DOPA and DOPAC levels were increased in some patients with pheochromocytoma and in all of 5 patients with neuroblastoma. We currently are developing a liquid chromatographic-radioimmunoassay (LC-RIA) for atrial natriuretic factor.

我们已经建立了测量血浆、尿液和 脑脊液中DOPA、DOPAC和儿茶酚胺的水平， 液相色谱电化学检测 的一个来源 分析中的污染峰是二氢咖啡酸，其是 咖啡酸是咖啡中的儿茶酚。 DOPA和DOPAC水平 在嗜铬细胞瘤患者中， 神经母细胞瘤患者。 我们目前正在开发一种液体 心钠素的色谱-放射免疫测定法（LC-RIA）。