SPECTROFLUOROMETRIC MEASUREMENT OF THE CRITICAL BILAYER ASSEMBLY TEMPERATURE T

双层组装临界温度 T 的荧光分光光度测量

• 批准号: 5204136
• 负责人: T L TALBOT
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5204136
• 关键词: biomedical equipment development; lipid bilayer membrane; membrane biogenesis; microspectrophotometry; thermodynamics

The study of the molecular mechanisms used by cells for the assembly of membranes is the principal focus of the instrumentation being developed in this project. The lipid bilayer of membranes has been shown to self-assemble at a critical point, T*, which is identical to the physiological temperature, previously measured by equilibrium thermodynmamic methods. Evaluation of T* under equilibrium conditions has been limited by the intrinsic slowness of the bilayer assembly processes at the critical point. Thus a measurement of T* is limited by the speed at which equilibrium is reached, and may take several weeks to complete. We have developed a scanning method for measuring T* that obviates the need to wait for equilibrium and allows a complete T* measurement in several hours. This method is now being implemented for large scale measurement of membrane samples for the detection of lipid-based metabolic defects in neurological tissues.

对细胞组装所用分子机制的研究 膜是仪器的主要焦点， 在这个项目中开发的。膜的脂双层已经被 显示在临界点T* 自组装，这与 先前通过平衡测量的生理温度 热力学方法平衡条件下T* 的计算 受到双层组装固有的缓慢性的限制 在临界点的过程。因此，T* 的测量是有限的 以达到平衡的速度，可能需要几个 周完成。我们开发了一种扫描测量方法 T*，避免了需要等待均衡，并允许 在几个小时内完成T* 测量。这种方法目前正在 实施用于膜样品的大规模测量 检测神经组织中基于脂质的代谢缺陷。