CENTER FOR BIOMEDICAL MASS SPECTROMETRY

巨大的技术进步导致了新的基因产物的发现，毫无疑问，蛋白质在致癌过程中的功能和机制的研究将自然而然地进行，并将需要灵敏的新分析工具。由于新的电离技术的无与伦比的敏感性和对生物聚合物进行测序的潜力，质谱学将在这些测定中发挥关键作用。这项提案要求在医学院建立一个生物医学质谱学(CBMS)中心，这将极大地促进十位著名研究人员在化学致癌、DNA修复、线粒体/核蛋白和细胞信号机制方面的研究计划。新收购的最先进的仪器包括电喷雾电离LC/MS和LC/MS/MS系统，以及基质辅助激光解吸。训练有素的质谱学家将直接与高级调查人员及其小组成员合作。将开展一些项目来进行非标准分析，这些分析需要大量发展实验参数和技术。将新技术纳入质谱学的前沿对这些调查人员至关重要，并将由高级人员进行。描述了一个行政结构，该结构将确保一个功能齐全的中心，具有最佳的沟通和所有NCI支持的调查人员的访问权限。此外，该中心将通过一系列关于生物医学应用和新技术的研讨会，并通过向研究生和本科生提供质谱学技术指导，在基础生物医学科学家的教育方面发挥领导作用。

Tremendous technical advances have led to the discovery of new gene products, and the natural progression to the investigation of protein function and mechanism in carcinogenesis will unquestionably follow and will require sensitive new analytical tools. Mass spectrometry will have a critical role in these determinations due to the unparalleled sensitivity of new ionization techniques and the potential for sequencing biopolymers. This proposal requests funds to establish a Center for Biomedical Mass Spectrometry (CBMS) in the School of Medicine that will greatly facilitate the research programs of ten eminent investigators working in chemical carcinogenesis, DNA repair, mitochondrial/nuclear proteins, and cell signaling mechanisms. Newly acquired state-of-the-art instrumentation is available including systems for electrospray ionization LC/MS and LC/MS/MS, and matrix assisted laser desorption. Trained mass spectrometrists will work directly with senior investigators and their group members. Projects will be undertaken to perform non-standard analyses that require considerable development of experimental parameters and techniques. Incorporation of new technologies at the cutting edge of mass spectrometry will be essential for these investigators and will be undertaken by senior personnel. An administrative structure is described that will ensure a fully functioning Center with optimal communication and with access for all NCI-supported investigators. In addition, the Center will provide leadership in the education of basic biomedical scientists through a series of seminars on biomedical applications and new technologies and by providing instruction in mass spectrometry techniques to students in graduate and undergraduate courses.