CENTER FOR BIOMEDICAL MASS SPECTROMETRY

巨大的技术进步导致了新的基因产物的发现，毫无疑问，对癌症发生过程中蛋白质功能和机制的研究将自然发展，并将需要灵敏的新分析工具。由于新的电离技术无与伦比的灵敏度和测序生物聚合物的潜力，质谱法将在这些测定中发挥关键作用。该提案要求在医学院建立生物医学质谱中心（CBMS），该中心将极大地促进10位杰出研究人员在化学致癌，DNA修复，线粒体/核蛋白和细胞信号传导机制方面的研究项目。新获得的最先进的仪器包括电喷雾电离LC/MS和LC/MS/MS系统，以及矩阵辅助激光解吸。训练有素的质谱师将直接与高级调查人员及其小组成员一起工作。项目将进行非标准分析，这需要相当大的实验参数和技术的发展。对这些研究人员来说，在质谱分析的前沿纳入新技术将是必不可少的，并将由高级人员承担。描述了一个行政结构，将确保一个功能齐全的中心，具有最佳的通信和所有nci支持的调查人员的访问权限。此外，该中心还将通过举办一系列关于生物医学应用和新技术的研讨会，以及为研究生和本科生提供质谱技术方面的指导，在基础生物医学科学家的教育方面发挥领导作用。

Tremendous technical advances have led to the discovery of new gene products, and the natural progression to the investigation of protein function and mechanism in carcinogenesis will unquestionably follow and will require sensitive new analytical tools. Mass spectrometry will have a critical role in these determinations due to the unparalleled sensitivity of new ionization techniques and the potential for sequencing biopolymers. This proposal requests funds to establish a Center for Biomedical Mass Spectrometry (CBMS) in the School of Medicine that will greatly facilitate the research programs of ten eminent investigators working in chemical carcinogenesis, DNA repair, mitochondrial/nuclear proteins, and cell signaling mechanisms. Newly acquired state-of-the-art instrumentation is available including systems for electrospray ionization LC/MS and LC/MS/MS, and matrix assisted laser desorption. Trained mass spectrometrists will work directly with senior investigators and their group members. Projects will be undertaken to perform non-standard analyses that require considerable development of experimental parameters and techniques. Incorporation of new technologies at the cutting edge of mass spectrometry will be essential for these investigators and will be undertaken by senior personnel. An administrative structure is described that will ensure a fully functioning Center with optimal communication and with access for all NCI-supported investigators. In addition, the Center will provide leadership in the education of basic biomedical scientists through a series of seminars on biomedical applications and new technologies and by providing instruction in mass spectrometry techniques to students in graduate and undergraduate courses.