CORE--CHEMISTRY

描述：（申请人描述）核心组分的主要目的是(1)建立集中的化学分析和寡脱氧核苷酸合成设施；(2)组织一系列研讨会，邀请特邀演讲者介绍与项目成员兴趣相关的研究；(3)为计划参与者提供行政支持。首席研究员协调项目的行政方面，召集咨询委员会，安排系列研讨会，并促进参与者之间的沟通。核心项目的科学功能由Iden博士指导，包括复杂的HPLC和质谱仪分析以及自动DNA合成和纯化。质谱法将在分析含有新DNA加合物和合成中间体的寡脱氧核苷酸中发挥关键作用。两个重要的新仪器，Micromass Quattro LC/MS/MS系统和Micromass Platform LC/MS仪器，最近被添加到质谱分析设施，将成为这些分析的基础。低聚脱氧核苷酸的合成和纯化对该计划的所有研究人员至关重要；这是最有效地完成作为一个集中的设施。在核心设施中包括研究的分析和综合方面，确保所有项目研究都可以免费使用。

DESCRIPTION: (APPLICANT'S DESCRIPTION) The primary objectives of the core component are (1) to establish centralized facilities for chemical analysis and oligodeoxynucleotide synthesis; (2) to organize a seminar series for invited speakers to present research related to the interests of Program members; and (3) to provide administrative support for Program participants. The Principal Investigator coordinates administrative aspects of the Program, convenes the Advisory Committee, arranges the seminar series, and facilitates communication between the participants. The scientific functions of the Core Project are directed by Dr. Iden and include sophisticated HPLC and mass spectrometer analysis and automated DNA synthesis and purification. Mass spectrometry will play a critical role in the analysis of oligodeoxynucleotides containing new DNA adducts and synthetic intermediates. Two important new instruments, a Micromass Quattro LC/MS/MS system and a Micromass Platform LC/MS instrument, were recently added to the Mass Spectrometry Facility and will be fundamental for these analyses. Synthesis and purification of oligodeoxynucleotides is of essential importance for all investigators in the Program; this is most effectively accomplished as a centralized facility. Including the analytical and synthetic aspects of the research in the core facility ensures that it is freely available for all project research.