EFFECT OF ANTIMICROBIAL COMBINATIONS ON CARIOGENIC MICROFLORA

抗菌药物组合对致龋微生物群的影响

• 批准号: 6238564
• 负责人: DAVID Ray DRAKE
• 金额: $ 19.22万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1998-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6238564
• 关键词: Streptococcus mutans; antibacterial agents; chemoprevention; chlorhexidine; combination chemotherapy; copper; dental caries inhibitor; dental plaque; fluorine; glucans; host organism interaction; human subject; lectin; microorganism culture; microorganism growth; oral bacteria; pyrophosphates

Antimicrobial strategies in the past have focused on reducing plaque overall and inhibiting its reformation to prevent caries. However, it is now known that the development of carious lesions is an intricate process involving microbial specificity (the mutans streptococci) and a multitude of dietary and host-derived factors. The 'ecological plaque hypothesis' embraces these interplaying components by proposing that one or more of these factors triggers a shift in proportions of plaque organisms thereby creating a more cariogenic flora. Therefore, a more rational approach in caries prevention would focus on inhibiting these deleterious shifts in microbial communities where the mutans streptococci become dominant. Our central hypothesis that exposure of the mutans streptococci to combinations of a metal ion (copper-Cu++) and chlorhexidine (CHX), or pyrophosphate (PPi) with sodium dodecyl sulfate (SDS) and fluoride (F), will affect the ability of these organisms to colonize teeth. The Specific Aims of this proposal are: 1. Assess the antimicrobial activity of two different combinations of antimicrobials: (a) CHX-Cu++, (b) PPi-SDS-F. 2. Determine the effect of subMIC concentrations of CHX-Cu++ and PPi- SDS-F on (a) expression of phenotypically-active glucan-binding lectins of the mutans streptococci, and (b) the ability of these organisms to adhere and colonize glucan-coated surfaces. 3. Delineate the effects of CHX-Cu++ and PPi-SDS-F combinations pulsed (high initial concentration) or dosed (low initial concentration gradually increased) on communities of oral bacteria grown in continuous culture. 4. Determine the effect of CHX-Cu++ and PPi-SDS-F combinations on plaque development and demineralization of enamel sections in an intraoral appliance model. The results from this grant will provide important information on these antimicrobial combinations and their potential anti-caries properties.

过去的抗菌策略主要集中在减少菌斑。 全面抑制其改造，预防龋病。然而，它 现在已知，龋齿病变的发展是一个错综复杂的过程 涉及微生物专一性的过程(变形链球菌)和 多种饮食和宿主来源的因素。“生态牌匾” 假说包含了这些相互作用的组成部分，提出了一个 这些因素中的更多因素会引发斑块比例的变化 从而创造出更具致龋性的菌群。因此，更多的 预防龋病的合理途径将集中在抑制这些方面 变形链球菌所在微生物群落的有害变化 成为统治者。 我们的中心假设是变形链球菌暴露在 金属离子(铜-铜++)和洗必泰(CHX)的组合，或 焦磷酸盐(PPI)与十二烷基硫酸钠(十二烷基硫酸钠)和氟(F)， 会影响这些生物在牙齿上定居的能力。这个 这项建议的具体目标是： 1.评估两种不同组合的抗菌剂的抗菌活性 抗菌药物：(A)CHX-铜++，(B)PPI-SDS-F。 2.确定亚MIC浓度对CHX-Cu++和PPI-的影响 SDS-F对(A)表型活性葡聚糖结合凝集素的表达 和(B)这些生物的能力 附着并定植涂有葡聚糖的表面。 3.描述了CHX-Cu++和PPI-SDS-F组合的脉冲效应 (高初始浓度)或加药(低初始浓度 逐渐增加)在连续生长的口腔细菌群落上 文化。 4.确定CHX-Cu++和PPI-SDS-F组合对斑块的影响 口腔内釉质切片的发育和脱矿 设备型号。 这笔赠款的结果将提供有关这些方面的重要信息 抗菌剂组合物及其潜在的防龋性。