EFFECT OF ANTIMICROBIAL COMBINATIONS ON CARIOGENIC MICROFLORA

过去的抗菌策略主要集中在减少牙菌斑上 全面抑制其再形成，预防龋病。 但 现在我们知道，龋损的发展是一个复杂的过程， 涉及微生物特异性（变形链球菌）和 多种饮食和宿主衍生因素。 生态牌匾 假说“包含了这些相互作用的组成部分，提出了一个 这些因素中的一个或多个触发了斑块比例的变化， 从而产生更易致龋的植物群。 因此， 在龋病预防中，合理的方法应侧重于抑制这些 微生物群落的有害变化， 成为主导。 我们的中心假设是变形链球菌暴露于 金属离子（铜-Cu ++）和氯己定（CHX）的组合，或 焦磷酸盐（PPi）与十二烷基硫酸钠（SDS）和氟化物（F）， 会影响这些微生物在牙齿上的繁殖能力 的 该提案的具体目标是： 1. 评估两种不同组合的抗菌活性 抗菌剂：（a）CHX-Cu++，（B）PPi-SDS-F。 2. 测定CHX-Cu++和PPi-的亚MIC浓度的影响。 SDS-F对（a）表型活性葡聚糖结合凝集素表达的影响 （B）这些生物体的能力， 粘附并定植在葡聚糖涂层表面。 3. 描述CHX-Cu++和PPi-SDS-F组合脉冲的影响 (high初始浓度）或投加（低初始浓度 逐渐增加）对口腔细菌群落的影响， 文化 4. 确定CHX-Cu++和PPi-SDS-F组合对菌斑的影响 口腔内釉质切片的发育和脱矿 设备型号。 这项赠款的结果将提供有关这些问题的重要信息。 抗微生物组合及其潜在的抗龋齿性质。

Antimicrobial strategies in the past have focused on reducing plaque overall and inhibiting its reformation to prevent caries. However, it is now known that the development of carious lesions is an intricate process involving microbial specificity (the mutans streptococci) and a multitude of dietary and host-derived factors. The 'ecological plaque hypothesis' embraces these interplaying components by proposing that one or more of these factors triggers a shift in proportions of plaque organisms thereby creating a more cariogenic flora. Therefore, a more rational approach in caries prevention would focus on inhibiting these deleterious shifts in microbial communities where the mutans streptococci become dominant. Our central hypothesis that exposure of the mutans streptococci to combinations of a metal ion (copper-Cu++) and chlorhexidine (CHX), or pyrophosphate (PPi) with sodium dodecyl sulfate (SDS) and fluoride (F), will affect the ability of these organisms to colonize teeth. The Specific Aims of this proposal are: 1. Assess the antimicrobial activity of two different combinations of antimicrobials: (a) CHX-Cu++, (b) PPi-SDS-F. 2. Determine the effect of subMIC concentrations of CHX-Cu++ and PPi- SDS-F on (a) expression of phenotypically-active glucan-binding lectins of the mutans streptococci, and (b) the ability of these organisms to adhere and colonize glucan-coated surfaces. 3. Delineate the effects of CHX-Cu++ and PPi-SDS-F combinations pulsed (high initial concentration) or dosed (low initial concentration gradually increased) on communities of oral bacteria grown in continuous culture. 4. Determine the effect of CHX-Cu++ and PPi-SDS-F combinations on plaque development and demineralization of enamel sections in an intraoral appliance model. The results from this grant will provide important information on these antimicrobial combinations and their potential anti-caries properties.