Streptococcus mutans and Dental Caries in Native American Children

变形链球菌和美国原住民儿童的龋齿

• 批准号: 8053823
• 负责人: DAVID Ray DRAKE
• 金额: $ 63.95万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-12 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8053823
• 关键词: 4 year old; 5 year old; Acids; Affect; African American; Age; Age-Months; American Indian and Alaska Native; Area; Asthma; Bacteria; Bacterial Infections; Behavior; Behavioral; Biological Assay; Caring; Chairperson; Child; Childhood; Chlorhexidine; Chronic Disease; Communities; Complex; DNA Fingerprinting; Data; Dental; Dental Hygiene; Dental Hygienists; Dental caries; Dentists; Development; Diet; Diet Records; Disease; Eating; Environmental Exposure; Environmental Risk Factor; Ethnic Origin; Etiology; Fluorides; Gel; Genetic Techniques; Glucans; Glucosyltransferase; Goals; Growth; Health; Health education; Height; Hour; Incidence; Infant; Infection; Intervention; Learning; Measurement; Microbial Biofilms; Modeling; Molecular Genetics; Mothers; National Health and Nutrition Examination Survey; Native Americans; Newborn Infant; Nutritional; Pain; Physiological; Population; Population Group; Poverty; Preschool Child; Prevalence; Prevention; Prevention strategy; Process; Property; Questionnaires; Race; Recording of previous events; Recruitment Activity; Research; Research Personnel; Risk; Risk Factors; Sampling; Severities; Siblings; Sioux Indians; Source; South Dakota; Staging; Streptococcus mutans; Structure; Surveys; Time; Toddler; Training; United States; Varnish; Vertical Disease Transmission; Virulence; Virulent; Water; Water fluoridation; Weight; Work; age group; antimicrobial; base; burden of illness; cariogenic bacteria; early childhood; high risk; preference; prevent; programs; transmission process; tribal community

DESCRIPTION (provided by applicant): Dental caries is the single most prevalent chronic disease of childhood. Early childhood caries (ECC), a particularly severe form of caries, affects infants, toddlers and preschool children younger than 6 years. Among the general U.S. population, the prevalence of ECC is 25% among children 2-5 years (NHANES III). More recently, NHANES data from 1999-2002 found that the prevalence of caries in young children had increased from the 1988-94 time period, from 24% to 28% in 2- to 5-year-old children (Beltran-Aguilar, et al, 2005). As in the previous surveys, the 1999-2002 NHANES data revealed demonstrated profound disparities in Dental caries prevalence and severity based on race/ethnicity and poverty status. Among American Indian and Alaska Native (AI/AN) children, however, the disease burden is much higher, with 79% of 2-5 year old AI/AN children having ECC (IHS, 2001). The reason for this disparity is not understood, partially because a comprehensive study on the etiology of ECC in this high-risk population has not been undertaken. Until the etiology of the disease is fully understood, there is little hope of developing effective preventive strategies. The overall goal of this study is to identify risk factors for ECC among AI/AN infants and toddlers and to determine if Streptococcus mutans (SM) alone, or in combination with environmental and behavioral factors, increases risk of caries in Native American children. The Specific Aims are: 1) To determine the temporality and fidelity of transmission of Streptococcus mutans in Native American children, 2) To characterize expression of virulence determinants of specific clones of Streptococcus mutans, 3) To determine the composition of total cultivable flora, total Streptococcus mutans and total acid flora in plaque samples of Native American subjects over time, 4) To determine the incidence of ECC in Native American children through age 36 months, and 5) To identify dietary and nutritional risk factors for acquisition of virulent SM clones and ECC in Native American infants and toddlers. Working closely with the Health Board of the Aberdeen Area Tribal Chairman, we will work with the Oglala Sioux tribal community at Pine Ridge, South Dakota from which we will recruit 200 Native mother/child dyads within 1 month of delivery. Information on potential risk factors and caries status will be collected every 4 months until the child is 36 months. As part of our effort to train AI/AN researchers, we will hire a local program coordinator and Dental hygienists. We believe that this assessment of vertical transmission of specific SM clones using DNA fingerprinting approaches will provide valuable information on the acquisition of these cariogenic bacteria in these Native American children.

描述（由申请人提供）：龋齿是儿童期最普遍的慢性疾病。幼儿龋齿（ECC）是一种特别严重的龋齿形式，影响6岁以下的婴儿、幼儿和学龄前儿童。在美国普通人群中，2-5岁儿童的ECC患病率为2- 5%（NHANES III）。最近，1999-2002年的NHANES数据发现，幼儿龋齿的患病率从1988-94年期间增加，2- 5岁儿童的龋齿患病率从24%增加到28%（Beltran-Aguilar等人，2005）。与以前的调查一样，1999-2002年全国健康和营养状况调查数据显示，龋齿患病率和严重程度因种族/民族和贫困状况而存在巨大差异。然而，在美洲印第安人和阿拉斯加原住民（AI/AN）儿童中，疾病负担要高得多，79%的2-5岁AI/AN儿童患有ECC（IHS，2001年）。这种差异的原因尚不清楚，部分原因是尚未对这一高危人群中ECC的病因进行全面研究。在充分了解该病的病因之前，制定有效的预防战略的希望不大。本研究的总体目标是确定AI/AN婴儿和幼儿中ECC的风险因素，并确定变形链球菌（SM）单独或与环境和行为因素结合是否会增加美国原住民儿童的龋齿风险。具体目标是：1）确定变形链球菌在美洲土著儿童中传播的时间性和保真度，2）表征变形链球菌特异性克隆的毒力决定子的表达，3）确定美洲土著受试者的牙菌斑样品中总可培养植物群、总变形链球菌和总酸性植物群随时间的组成，4）确定36个月内美国土著儿童ECC的发病率，5）确定美国土著婴儿和幼儿获得有毒SM克隆和ECC的饮食和营养风险因素。我们将与阿伯丁地区部落主席卫生委员会密切合作，与南达科他州松岭的奥格拉拉苏族部落社区合作，在分娩后1个月内招募200名土著母亲/儿童。有关潜在风险因素和龋齿状况的信息将每4个月收集一次，直到孩子36个月。作为我们培训AI/AN研究人员的努力的一部分，我们将聘请当地的项目协调员和牙科医生。我们相信，这种评估特定SM克隆的垂直传播，使用DNA指纹图谱的方法将提供有价值的信息，这些致龋细菌在这些美洲土著儿童的收购。

项目成果

Dental caries in a cohort of very young American Indian children.

一群非常年幼的美国印第安儿童的龋齿。

• DOI: 10.1111/j.1752-7325.2012.00372.x
• 发表时间: 2012
• 期刊: Journal of public health dentistry
• 影响因子: 2.3
• 作者: Warren,JohnJ;Kramer,KatherineWO;Phipps,Kathy;Starr,Delores;Dawson,DeborahV;Marshall,Teresa;Drake,David
• 通讯作者: Drake,David

Improved method for rapid and accurate isolation and identification of Streptococcus mutans and Streptococcus sobrinus from human plaque samples.

从人类菌斑样本中快速准确分离和鉴定变形链球菌和远缘链球菌的改进方法。

• DOI: 10.1016/j.mimet.2017.06.009
• 发表时间: 2017
• 期刊: Journal of microbiological methods
• 影响因子: 2.2
• 作者: Villhauer,AlissaL;Lynch,DavidJ;Drake,DavidR
• 通讯作者: Drake,DavidR

Genotypic characterization and comparison of Streptococcus mutans in American Indian and Southeast Iowa children.

美洲印第安人和爱荷华州东南部儿童中变形链球菌的基因型特征和比较。

• DOI: 10.1002/cre2.94
• 发表时间: 2017
• 期刊: Clinical and experimental dental research
• 影响因子: 1.8
• 作者: Villhauer,AlissaL;Lynch,DavidJ;Warren,JohnJ;Dawson,DeborahV;Blanchette,DerekR;Drake,DavidR
• 通讯作者: Drake,DavidR

Experiences with the Streptococcus Mutans in Lakota Sioux (SMILeS) Study: Risk factors for Caries in American Indian Children 0-3 Years.

拉科塔苏族变形链球菌 (SMILeS) 研究的经验：美洲印第安人 0-3 岁儿童龋齿的危险因素。

• 发表时间: 2015
• 期刊: Journal of health disparities research and practice
• 作者: Drake,David;Dawson,Deborah;Kramer,Katherine;Schumacher,Amy;Warren,John;Marshall,Teresa;Starr,Delores;Phipps,Kathy
• 通讯作者: Phipps,Kathy

Evidence of Early Emergence of the Primary Dentition in a Northern Plains American Indian Population.

• DOI: 10.1177/2380084418756054
• 发表时间: 2018-04-01
• 期刊: JDR clinical and translational research
• 作者: Dawson, D V;Blanchette, D R;Drake, D R
• 通讯作者: Drake, D R