COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS (GELLAB)

计算机辅助二维电泳凝胶分析 (GELLAB)

• 批准号: 6289201
• 负责人: peter f lemkin
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6289201
• 关键词: Rett syndrome; computer assisted diagnosis; computer data analysis; computer network; computer program /software; computer system design /evaluation; gel electrophoresis; human data; human genetic material tag; image processing; information systems; mass screening; neoplasm /cancer classification /staging; nuclear matrix; prostate neoplasms; protein sequence; renal cell carcinoma

The GELLAB-II software system is an exploratory data analysis system for theanalysis of sets of 2D electrophoretic protein gel images. It incorporatessophisticated subsystems for image acquisition, processing, database manipulation, graphics and statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes,in one or more proteins among hundreds or thousands of unaltered proteins, isthe basic analytic problem. Keeping track of changes detected using thesemethods is also a major attribute of the system. A composite gel database maybe viewed under different exploratory data analysis conditions, andstatistical differences and subtle patterns elucidated from  slicesof an effective 3D database. Results can be presented in a variety of tables,plots or derived images and on workstations over wide area networks. TheGELLAB-II research has resulted in a technology transfer CRADA withCSPI/Scanalytics Inc., for a commercially available system GELLAB-II+, makingthis technology easily available to cancer researchers on inexpensive MicrosoftWindows PCs (released in 1996). Such commercialization resulted in wider use and better support of the GELLAB-II technology than we can provide. GELLAB-IIapplications this period include: Ongoing studies of nuclear matrix proteinchanges in prostate cancer to aid screening and staging of men with prostate cancer (J.Hopkins); and extending an earlier Rett syndrome study (CDC) RobinsonMK, Myrick J, etal., Electrophoresis 16: 1176-83, 1995. In the prostate cancerstudies, we are looking for both missing proteins as well as subtle quantitativechanges in patterns on sets of proteins which correlates with experimental conditions, staging and screening of men with prostate cancer. We are also doing a similar investigation of nuclear matrix protein patterns from renal cellcarcinoma and other genitourinary tissues. The Hopkins groups prostate molecular work has identified several of the protein spots by sequence analysisand are completing studies to confirm these sequences. Z01 BC 08381-16 - Chromatin Structure, databases, electrophoresis, gene expression, image analysis, Prostate cancer,

GELLAB-II软件系统是一个探索性的数据分析系统，用于分析2D电泳蛋白质凝胶图像集。它包含了用于图像采集、处理、数据库操作、图形和统计分析的复杂子系统。它已被应用于各种实验系统中，定量和定性的变化，在一个或多个蛋白质中的数百或数千个不变的蛋白质，是基本的分析问题。跟踪使用这些方法检测到的变化也是系统的一个主要属性。复合凝胶数据库可能 观看 在不同的探索性数据分析条件下，  切片有效的3D数据库。结果可以以各种表格、绘图或衍生图像的形式呈现，并通过广域网在工作站上呈现。GELLAB-II研究已经与CSPI/Scanalytics Inc.达成了技术转让CRADA，GELLAB-II+的商业化系统，使得癌症研究人员可以在廉价的MicrosoftWindows PC（1996年发布）上轻松使用这项技术。这种商业化导致了GELLAB-II技术的更广泛的使用和更好的支持，超过了我们所能提供的。GELLAB-II在这一时期的应用包括：正在进行的前列腺癌核基质蛋白变化的研究，以帮助前列腺癌男性的筛查和分期（J.霍普金斯）;和扩展早期的Rett综合征研究（CDC）RobinsonMK，Myrick J埃塔尔，电泳16：1176-83，1995。在前列腺癌研究中，我们正在寻找缺失的蛋白质以及与前列腺癌患者的实验条件、分期和筛查相关的蛋白质组模式的细微定量变化。我们也在对肾细胞癌和其他泌尿生殖系统组织的核基质蛋白模式进行类似的研究。霍普金斯小组的前列腺分子工作已经通过序列分析鉴定了几个蛋白质点，并正在完成对这些序列的确认研究。Z 01 BC 08381-16 -染色质结构，数据库，电泳，基因表达，图像分析，前列腺癌，