Interactive Biomedical Image Flicker-Comparison Across the Internet

世界各地的科学家经常研究类似的数据，因此需要定期分享结果和比较数据。 我们描述了一种闪烁比较方法，比较在不同实验室创建的两个相似样品的图像，以帮助识别或建议蛋白质点识别。 由于2D凝胶和相关数据库（DB）经常出现在互联网上，这就开辟了将自己的实验2D凝胶图像数据与远程互联网DB中的另一凝胶数据进行视觉比较的可能性。 一般来说，有几种方法可以比较图像：1）将一种放射自显影或染色的凝胶在另一种凝胶上滑动，同时背光;或2）在扫描和分析这些凝胶后，从两种凝胶构建2D凝胶计算机DB。 这些是不切实际的，因为在前一种情况下，来自互联网DB的凝胶在本地不可用，而如果仅需要单个视觉比较，则构建完整DB的成本过高。 我们描述了一个分布式凝胶比较程序（http：//www-lecb.ncifcrf.gov/flicker），它可以在任何Web浏览器上运行，并通过点击Web浏览器的请求来调用。 一个图像从任何互联网2D凝胶DB（例如SWISS-2DPAGE）读取，另一个从研究者的计算机读取。通过首先应用空间扭曲或其他变换，可以更容易地比较图像。 由于两个可能变换的图像在同一窗口中快速交替或闪烁，因此用户可以将一个图像滑过另一个图像，以通过匹配局部形态来在视觉上对准对应的斑点或区域。这种闪烁比较技术可以应用于分析其他类型的1D和2D生物医学图像。 我们已经开发了一个2DWG元数据库的2D凝胶图像在网络上找到，并将其与闪烁，使其更容易比较图像（http：www-lecb.ncifcrf.gov/2dwgDB/）。Z01 BC 10044-04

Scientists around the world often work on similar data so the need to share results and compare data arises periodically. We describe a flicker-comparison method of comparing image of two similar samples created in different laboratories to help identify or suggest protein spot identification. Now that 2D gels and associated databases (DB) frequently appear on the Internet, this opens up the possibility of visually comparing one's own experimental 2D gel image data with data from another gel in a remote Internet DB. In general, there are a few ways to compare images: 1) slide one autoradiograph or stained gel over the other while back-illuminated; or 2) build a 2D gel computer DB from both gels after scanning and analyzing these gels. These are impractical since in the former case, the gel from the Internet DB is not locally available, while the costs of building a full DB is excessive if only a single visual comparison is needed. We describe a distributed gel comparison program (http://www-lecb.ncifcrf.gov/flicker) which runs on any Web browser and is invoked by clicking on a request from the web browser. One image is read from any Internet 2D gel DB (eg.SWISS-2DPAGE) and the other from the investigator's computer. Images may be more easily compared by first applying spatial warping or other transforms. As the two possibly transformed images are rapidly alternated, or flickered, in the same window, the user can slide one image past the other to visually align corresponding spots or regions by matching local morphology. This flicker-comparison technique may be applied to analyzing other types of 1D and 2D biomedical images. We have developed a 2DWG meta-database of 2D gel images found on the Web and integrated it with Flicker to make it easier to compare images (http:www-lecb.ncifcrf.gov/2dwgDB/). Z01 BC 10044-04