Exploratory Data Analysis of Web Quantitative Gel Databa

早期的二维凝胶数据分析系统需要一台专用的独立计算机和系统人员来安装和运行特殊的二维凝胶软件。我们一直有兴趣将凝胶分析软件的访问权限扩展到协作小组设置，以便所有小组成员无论在哪里都可以处理最新的数据。我们扩展了WebGel系统，允许合作者通过互联网探索性地访问GELLAB-II、GELLAB-II(Scanalytics)、Melanie-II和其他2D和1D Gel数据库。用户可以通过Web浏览器访问在工作站站点准备并复制到Web服务器上的数据。他们可以进行各种数据探索，包括点归一化和凝胶组之间相应点的统计比较。(对于一维凝胶，斑点将被条带取代。)他们可以一次用Flicker比较两种凝胶，找出显著变化的斑点，然后用WebGel(http：//www-lecb.ncifcrf.gov/WebGel/)查找相应的定量数据。WebGel由一组相互依赖的HTML、CGI-BIN、Java小程序和JavaScript程序(用于构建网页的编程语言)组成，用于访问部分传统GELLAB-II定量数据。用户可以看到单一的Web界面，并且可以透明地访问他们自己和他们的合作者的数据视图。我们一直在用它来分析前列腺癌肿瘤标志物凝胶中的核基质蛋白(Partin，et.Al)、磷蛋白(Hornbeck)、胎儿酒精综合征凝胶(Myrick)和2D DNA凝胶中癌基因的DNA变化(Patrick)。 Z01公元前10259-03

Early 2D gel data analysis systems required a dedicated stand-alone computer and systems people to install and run the special 2D gel software. We have been interested in extending the access of gel analysis software to a collaborative group setting so that all group members can work on the latest data wherever they are physically located. We have expanded the WebGel system to allow exploratory access of GELLAB-II, GELLAB-II+ (Scanalytics), Melanie-II and other 2D and 1D gel databases to collaborators via the Internet. Users can access their data prepared at the workstation site and copied to their Web server through a Web browser. They can perform various data explorations including spot normalization and statistical comparison of corresponding spots across sets of gels. (For 1D gels, spots would be replaced by bands.) They can compare two gels at a time with Flicker to find significantly changing spots and then look up the corresponding quantitative data with WebGel (http:/www-lecb.ncifcrf.gov/webgel/). WebGel consists of an interdependent set of HTML, CGI-BIN, Java applets, and JavaScript programs (programming languages used to construct Web pages) to access parts of the legacy GELLAB-II quantitative data. The user sees a single Web interface and has transparent access to their own and their collaborators' views of the data. We have been using it to analyze nuclear matrix proteins in prostate cancer gels for tumor markers (Partin, et. al), phosphoproteins (Hornbeck), fetal alcohol syndrome gels (Myrick), and DNA alternations of oncogenes in 2D DNA gels (Patrick). Z01 BC 10259-03