EXPLORATORY DATA ANALYSIS OF WEB QUANTITATIVE GEL DATABASES

早期的2D凝胶数据分析系统需要专用的独立计算机和系统人员来安装和运行特殊的2D凝胶软件。我们一直有兴趣将凝胶分析软件的访问扩展到协作组设置，以便所有组成员无论身在何处都可以处理最新的数据。我们已经扩展了WebGel系统，允许合作者通过互联网探索访问GELLAB-II， GELLAB-II+ (Scanalytics)， Melanie-II和其他2D和1D凝胶数据库。用户可以通过Web浏览器访问在工作站站点准备并复制到其Web服务器的数据。他们可以进行各种数据探索，包括斑点归一化和凝胶组中相应斑点的统计比较。（对于1D凝胶，斑点将被条带取代。）他们可以用Flicker一次比较两种凝胶，找到显著变化的点，然后用WebGel查找相应的定量数据。WebGel由一组相互依赖的HTML、CGI-BIN、Java applet和JavaScript程序（用于构建Web页面的编程语言）组成，以访问部分遗留的GELLAB-II定量数据。用户看到一个单一的Web界面，并且可以透明地访问他们自己和协作者的数据视图。我们一直在用它来分析前列腺癌凝胶中的核基质蛋白，用于肿瘤标志物（Partin等人），磷酸化蛋白（Hornbeck），胎儿酒精综合征凝胶（Myrick），以及二维DNA凝胶中致癌基因的DNA改变（Patrick）。Web，数据挖掘，2DE凝胶，蛋白质，人体组织，液体，细胞等。

Early 2D gel data analysis systems required a dedicated stand-alone computer and systems people to install and run the special 2D gel software. We have been interested in extending the access of gel analysis software to a collaborative group setting so that all group members can work on the latest data wherever they are physically located. We have expanded the WebGel system to allow exploratory access of GELLAB-II, GELLAB-II+ (Scanalytics), Melanie-II and other 2D and 1D gel databases to collaborators via the Internet. Users can access their data prepared at the workstation site and copied to their Web server through a Web browser. They can perform various data explorations including spot normalization and statistical comparison of corresponding spots across sets of gels. (For 1D gels, spots would be replaced by bands.) They can compare two gels at a time with Flicker to find significantly changing spots and then look up the corresponding quantitative data with WebGel. WebGel consists of an interdependent set of HTML, CGI-BIN, Java applets, and JavaScript programs (programming languages used to construct Web pages) to access parts of the legacy GELLAB-II quantitative data. The user sees a single Web interface and has transparent access to their own and their collaborators views of the data. We have been using it to analyze nuclear matrix proteins in prostate cancer gels for tumor markers (Partin, et. al), phosphoproteins (Hornbeck), fetal alcohol syndrome gels (Myrick), and DNA alternations of oncogenes in 2D DNA gels (Patrick).Z01 BC 10259-03 - Web, data mining, 2DE gels, proteins, - Human Tissues, Fluids, Cells, etc.