Interactive Biomedical Image Flicker-Comparison Across t

世界各地的科学家经常研究类似的数据，因此需要定期分享结果和比较数据。我们描述了一种闪烁比较方法，比较在不同实验室创建的两个相似样品的图像，以帮助识别或建议蛋白质斑点识别。现在二维凝胶和相关数据库（DB）经常出现在互联网上，这就提供了将自己的实验二维凝胶图像数据与远程互联网数据库中另一种凝胶的数据进行直观比较的可能性。一般来说，有几种比较图像的方法：1)在背光照射下，将一张自显影片或染色凝胶片放在另一张上；或2)在扫描和分析凝胶后，利用这两种凝胶构建二维凝胶计算机DB。这是不切实际的，因为在前一种情况下，来自Internet DB的凝胶不是本地可用的，而如果只需要单个视觉比较，则构建完整DB的成本过高。我们描述了一个分布式凝胶比较程序（http://www-lecb.ncifcrf.gov/flicker），它可以在任何Web浏览器上运行，并通过点击Web浏览器的请求来调用。一张图片从任何互联网2D凝胶数据库（例如：swiss - 2dpage）读取，另一张从研究者的计算机读取。通过首先应用空间扭曲或其他变换，图像可以更容易地进行比较。由于两个可能被转换的图像在同一窗口中快速交替或闪烁，用户可以将一个图像滑动到另一个图像之前，通过匹配局部形态学来视觉上对齐相应的点或区域。这种闪烁比较技术可以应用于分析其他类型的1D和2D生物医学图像。我们已经开发了一个2DWG元数据库，其中包含了在网络上发现的2D凝胶图像，并将其与Flicker集成在一起，以便更容易地比较图像（http:www-lecb.ncifcrf.gov/2dwgDB/）。

Scientists around the world often work on similar data so the need to share results and compare data arises periodically. We describe a flicker-comparison method of comparing image of two similar samples created in different laboratories to help identify or suggest protein spot identification. Now that 2D gels and associated databases (DB) frequently appear on the Internet, this opens up the possibility of visually comparing one's own experimental 2D gel image data with data from another gel in a remote Internet DB. In general, there are a few ways to compare images: 1) slide one autoradiograph or stained gel over the other while back-illuminated; or 2) build a 2D gel computer DB from both gels after scanning and analyzing these gels. These are impractical since in the former case, the gel from the Internet DB is not locally available, while the costs of building a full DB is excessive if only a single visual comparison is needed. We describe a distributed gel comparison program (http://www-lecb.ncifcrf.gov/flicker) which runs on any Web browser and is invoked by clicking on a request from the web browser. One image is read from any Internet 2D gel DB (eg.SWISS-2DPAGE) and the other from the investigator's computer. Images may be more easily compared by first applying spatial warping or other transforms. As the two possibly transformed images are rapidly alternated, or flickered, in the same window, the user can slide one image past the other to visually align corresponding spots or regions by matching local morphology. This flicker-comparison technique may be applied to analyzing other types of 1D and 2D biomedical images. We have developed a 2DWG meta-database of 2D gel images found on the Web and integrated it with Flicker to make it easier to compare images (http:www-lecb.ncifcrf.gov/2dwgDB/). Z01 BC 10044-04