INTERACTIVE BIOMEDICAL IMAGE FLICKER-COMPARISON ACROSS THE INTERNET

世界各地的科学家经常研究类似的数据，因此定期出现分享结果和比较数据的需求。我们描述了一种闪烁比较法，该方法比较在不同实验室创建的两个相似样本的图像，以帮助识别或建议蛋白质斑点识别。现在2D凝胶和相关数据库(DB)频繁地出现在互联网上，这打开了将自己的实验2D凝胶图像数据与远程互联网DB中的另一凝胶的数据进行可视比较的可能性。一般来说，有几种方法可以比较图像：1)在背光照射下，将一个放射自显像仪或染色凝胶滑动到另一个上；或2)在扫描和分析这些凝胶后，从这两个凝胶建立2D凝胶计算机DB。这些都是不切实际的，因为在前一种情况下，来自Internet数据库的凝胶在本地是不可用的，而如果只需要一个直观的比较，那么构建一个完整的数据库的成本是过高的。我们描述了一个分布式凝胶比较程序(网址：http://www-lecb.ncifcrf.gov/flicker)，它运行在任何网络浏览器上，并通过点击来自网络浏览器的请求来调用。一个图像从任何互联网2D Gel DB(例如SWISS-2DPAGE)读取，另一个图像从调查人员的计算机读取。通过首先应用空间扭曲或其他变换，可以更容易地比较图像。当两个可能变换的图像在同一窗口中快速交替或闪烁时，用户可以将一个图像滑过另一个图像以通过匹配局部形态在视觉上对准相应的点或区域。这种闪烁比较技术可以应用于分析其他类型的一维和二维生物医学图像。我们已经开发了一个在Web上找到的2D Gel图像的2DWG元数据库，并将其与Flicker集成在一起，以便更容易地比较图像(http：www.lecb.ncifcrf.gov/2dwgDB/)。Z01 BC 10044-04-癌症预防，数据库，电泳，基因表达，图像分析，可能的蛋白质鉴定，

Scientists around the world often work on similar data so the need to share results and compare data arises periodically. We describe a flicker-comparison method of comparing image of two similar samples created in different laboratories to help identify or suggest protein spot identification. Now that 2D gels and associated databases (DB) frequently appear on the Internet, this opens up the possibility of visually comparing ones own experimental 2D gel image data with data from another gel in a remote Internet DB. In general, there are a few ways to compare images: 1) slide one autoradiograph or stained gel over the other while back-illuminated; or 2) build a 2D gel computer DB from both gels after scanning and analyzing these gels. These are impractical since in the former case, the gel from the Internet DB is not locally available, while the costs of building a full DB is excessive if only a single visual comparison is needed. We describe a distributed gel comparison program (URL:http://www- lecb.ncifcrf.gov/flicker) which runs on any Web browser and is invoked by clicking on a request from the web browser. One image is read from any Internet 2D gel DB (eg.SWISS-2DPAGE) and the other from the investigators computer. Images may be more easily compared by first applying spatial warping or other transforms. As the two possibly transformed images are rapidly alternated, or flickered, in the same window, the user can slide one image past the other to visually align corresponding spots or regions by matching local morphology. This flicker-comparison technique may be applied to analyzing other types of 1D and 2D biomedical images. We have developed a 2DWG meta-database of 2D gel images found on the Web and integrated it with Flicker to make it easier to compare images (http:www-lecb.ncifcrf.gov/2dwgDB/). Z01 BC 10044-04 - Cancer Prevention, databases, electrophoresis, gene expression, image analysis, putative protein identification,