CARBOHYDRATE PRIMARY STRUCTURE BY NMR

通过 NMR 分析碳水化合物的一级结构

• 批准号: 6653575
• 负责人: JOHN GLUSHKA
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653575
• 关键词: biological products; biomedical resource; carbohydrates; nuclear magnetic resonance spectroscopy; nucleic acids; technology /technique

A synthetic nucleotide sugar was submitted for confirmation of its structure and generation of a standard 1H-NMR spectrum for company files. NMR analysis was performed on a Varian Mercury 300-MHz spectrometer. The sample was deuterium-exchanged by lyophilization from D2O and dissolved in 0.5 mL D2O. The 1H-NMR 1-D spectrum was obtained at 25(C using presaturation of the residual HOD signal. Chemical shifts were measured relative to an internal acetone standard ((=2.225 ppm). Purity was assessed, and the structure, including anomeric configuration, was confirmed by comparison with previously published spectral data for a synthetic nucleotide sugar of identical structure.

提交合成核苷酸糖以确认其 公司的标准1H-NMR频谱的结构和生成 文件。 NMR分析在Varian汞300-MHz上进行 光谱仪。 样品是通过冻干的氘化处理的 来自D2O并溶解在0.5 mL D2O中。 1H-NMR 1-D光谱是 在25处获得（使用残留HOD信号的天； 相对于内部丙酮标准测量化学位移 （（= 2.225 ppm）。评估纯度，结构，包括 通过与先前的比较证实了异构配置 已发布的光谱数据，用于相同的合成核苷酸糖 结构。