GLYCOSYL COMPOSITION ANALYSIS

糖基成分分析

• 批准号: 6653594
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653594
• 关键词: bacteria; bioengineering /biomedical engineering; biomedical resource; carbohydrates; environmental health; spectrometry

Glycosyl composition analysis was performed on a bacterial exopolysaccharide from Pseudomonas putida. The sample was analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The sample was then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using a Hewlett Packard 5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

糖基组成分析进行了细菌 恶臭假单胞菌胞外多糖。 分析样品 通过制备三甲基甲硅烷基， 甲基糖苷的衍生物，随后进行气相色谱分析 (GC)气相色谱-质谱联用（GC-MS） 分析. TMS甲基糖苷由样品制备， 在3 M HCl中甲醇分解，然后用吡啶N-乙酰化， 醋酸酐 然后用Tri-Sil处理样品。 GC TMS甲基糖苷的分析是在HP 5890 GC上使用 DB 1熔融石英毛细管柱。 GC-MS分析使用 连接到5970 MSD的Hewlett Packard 5890 GC。 单糖 通过与标准品相比的保留时间进行鉴别，以及 这些化合物碳水化合物的性质是通过它们的质量来鉴定的 谱