GLYCOSYL COMPOSITION ANALYSIS

糖基成分分析

• 批准号: 6653594
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653594
• 关键词: bacteria; bioengineering /biomedical engineering; biomedical resource; carbohydrates; environmental health; spectrometry

Glycosyl composition analysis was performed on a bacterial exopolysaccharide from Pseudomonas putida. The sample was analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The sample was then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using a Hewlett Packard 5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

对一株细菌进行了糖基组成分析 恶臭假单胞菌胞外多糖。对样品进行了分析 通过制备三甲基硅基来合成单糖 甲基糖苷类化合物的气相色谱 (GC)和气质联用(GC-MS) 分析。通过以下方法从样品中制备TMS甲基糖苷 在3M盐酸中甲烷化，然后与吡啶进行N-乙酰化反应，然后 醋酸酐。然后用Tri-sil处理样品。气相色谱 在HP5890色谱柱上，使用 DB1熔融石英毛细管柱。GC-MS分析使用 惠普5890 GC和5970 MSD。单糖是 通过与标准相比的保留时间来识别，以及 这些物质的碳水化合物特性通过它们的质量进行了鉴定 光谱。