GLYCOSYL COMPOSITION ANALYSIS OF GLYCOPROTEIN SAMPLES

糖蛋白样品的糖基组成分析

• 批准号: 6653593
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653593
• 关键词: biomedical resource; carbohydrates; plants; spectrometry

The sample was analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The samples were then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

通过通过 制备甲基糖苷的三甲基硅烷基衍生物 其次是气相色谱法（GC）和合并气体 色谱/质谱法（GC-MS）分析。 TMS 通过甲解析在3 m中从样品中制备甲基糖苷 HCl随后用吡啶和乙酸酸酐进行N-乙酰化。 这 然后将样品用Tri-Sil处理。 TMS的GC分析 使用DB1融合二氧化硅在HP5890 GC上进行甲基糖苷 毛细管柱。 使用HP5890 GC进行GC-MS分析 耦合到5970 MSD。 单糖通过他们的 与标准和碳水化合物相比，保留时间 它们的特征是通过其质谱来验证的。