GLYCOSYL COMPOSITION ANALYSIS OF SACCHARIDE

糖的糖基组成分析

• 批准号: 6653596
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653596
• 关键词: biological products; biomedical resource; carbohydrates; oral pharyngeal; spectrometry

Glycosyl-linkage analysis was performed by preparing permethylated alditiol acetates using the Hakomori procedure. The samples were dissolved in DMSO, DMSO anion was added, and the samples were stirred at room temperature for 2 h. An excess of methyliodide was added, and the samples were stirred again from 2 to 4 h at room temperature. Excess methyliodide was removed by evaporation, and the samples were purified using a C18 SepPak cartridge. The resulting samples were hydrolyzed in 2 M TFA, and the monosaccharides were reduced to their alditiols and N-acetylated using acidic anhydride in pyridine. The resulting acetates were then analyzed by GC-MS using a 30 mm SP2330 column from Supelco for the neutral sugars and using a DB1 column from J&W Scientific for the methylated amino sugars.

通过制备苄丙二酰化，进行糖基 - 连接分析 使用HAKOMORI程序的丙二醇乙酸。 样品是 溶解在DMSO中，添加DMSO阴离子，并搅拌样品 在室温2小时。添加了过量的甲基碘，并且 在室温下，样品再次从2到4小时搅拌。 通过蒸发去除去过多的甲基碘，样品是 使用C18 Seppak墨盒纯化。 由此产生的样本是 在2 M TFA中水解，单糖降低至其 使用吡啶中的酸性酸酐和N-乙酰化的醛醇。 这 然后使用30 mm SP2330通过GC-MS分析所得的醋酸盐 Supelco的列用于中性糖，并使用来自 J＆W科学用于甲基化氨基糖。