GLYCOSYL COMPOSITION ANALYSIS OF SACCHARIDE

糖的糖基组成分析

• 批准号: 6653596
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653596
• 关键词: biological products; biomedical resource; carbohydrates; oral pharyngeal; spectrometry

Glycosyl-linkage analysis was performed by preparing permethylated alditiol acetates using the Hakomori procedure. The samples were dissolved in DMSO, DMSO anion was added, and the samples were stirred at room temperature for 2 h. An excess of methyliodide was added, and the samples were stirred again from 2 to 4 h at room temperature. Excess methyliodide was removed by evaporation, and the samples were purified using a C18 SepPak cartridge. The resulting samples were hydrolyzed in 2 M TFA, and the monosaccharides were reduced to their alditiols and N-acetylated using acidic anhydride in pyridine. The resulting acetates were then analyzed by GC-MS using a 30 mm SP2330 column from Supelco for the neutral sugars and using a DB1 column from J&W Scientific for the methylated amino sugars.

通过制备全甲基化产物进行糖基键分析 采用Hakomori法合成乙酸乙二醇酯。样本是 溶解于DMSO，加入DMSO阴离子，搅拌样品 在室温下放置2小时。加入过量的亚甲基碘化物，以及 样品在室温下再次搅拌2~4h。 通过蒸发除去多余的亚甲基，并将样品 使用C18 SepPak色谱柱进行纯化。由此产生的样本是 在2M TFA中水解，单糖被还原为 醛二醇和N-乙酰化，用酸性酸酐在吡啶中。这个 然后使用30 mm SP2330用GC-MS分析生成的乙酸酯 来自Supelco的柱用于中性糖，并使用来自 强生科学公司的甲基化氨基糖。