GLYCOSYL COMPOSITION ANALYSIS OF LIPOPOLYSACCHARIDES FROM PREVOTELLA INTERMEDIA

中间普雷沃氏菌脂多糖的糖基组成分析

• 批准号: 6653604
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653604
• 关键词: biomedical resource; carbohydrates; plants; spectrometry

The samples were analyzed for monosaccharide composition by preparing the TMS derivatives of the methylglycosides followed by GC-MS analysis. The samples were acid hydrolyzed then subjected to methanolysis followed by N-acetylation with pyridine and acetic anhydride (for detection of amino sugars). The samples were then treated with Tri-Sil. GC-MS analysis of the TMS methylglycosides was performed on an HP5890 GC coupled to a 5970 MSD using a 30-meter DB1 fused silica capillary column. The samples were subjected to glycosyl-linkage analysis using the Hakomori methylation procedure followed by combined GC-MS analysis. The samples were then subjected to acid hydrolysis using 2 M trifluoroacetic acid to effect cleavage of all glycosidic linkages. The glycosyl residues were reduced with NaBD4 and then acetylated using acetic anhydride/pyridine. The resulting partially methylated alditol acetates were analyzed on a 30-meter Supelco 2330 fused silica capillary column b y GC-MS using an HP 5890 GC coupled to a 5970 MSD. The samples were analyzed by 1-D 1H-NMRspectroscopy on a Bruker AMX-500 instrument.

通过以下方法分析样品的单糖组成： 制备甲基糖苷的TMS衍生物， GC-MS分析。 将样品酸水解，然后进行 甲醇分解，然后用吡啶和乙酸进行N-乙酰化 酸酐（用于检测氨基糖）。 然后将样品 使用Tri-Sil治疗。 对TMS甲基糖苷进行GC-MS分析。 在与5970 MSD偶联的HP 5890 GC上使用30米DB 1 熔融石英毛细管柱 对样品进行 使用Hakomori甲基化程序的糖基连接分析 随后进行GC-MS分析。 然后对样品进行 使用2 M三氟乙酸进行酸水解以实现裂解 所有的糖苷键。 糖基残基被还原， NaBD 4，然后使用乙酸酐/吡啶乙酰化。 的 将得到的部分甲基化的糖醇乙酸酯在 30-米Supelco 2330熔融石英毛细管柱B y GC-MS，使用 HP 5890 GC与5970 MSD偶联。 样品进行一维分析 在Bruker AMX-500仪器上的1H-NMR光谱。