GLYCOSYL COMPOSITION ANALYSIS OF GLYCOPROTEINS

通过对样品进行单糖组成分析 甲基糖苷三甲基硅基衍生物的制备 其次是气相色谱(GC)和组合气体 色质联用(GC-MS)分析。TMS 样品经3M甲醇裂解制得甲基苷 然后用盐酸与吡啶和乙酸酐进行N-乙酰化。这个 样品用Tri-sil处理。TMS的气相色谱分析 甲基糖苷在HP5890 GC上使用DB1熔融硅胶进行 毛细管柱。使用HP5890 GC进行GC-MS分析 再配上5970MSD。单糖的鉴定是通过它们的 与标准相比的保留时间，以及碳水化合物 这些化合物的性质通过它们的质谱图进行了鉴定。

The sample was analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The sample was then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.