GLYCOSYL LINKAGE ANALYSIS OF WATER SOLUBLE & INSOLUBLE LINKED GLUCANS

水溶性的糖基连接分析

• 批准号: 6653599
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653599
• 关键词: biological products; biomedical resource; carbohydrates; drug /agent; spectrometry

The sample was permethylated and analyzed by MALDI-MS using a 2,5-dihydroxybenzoic acid matrix. Methylation of the sample was accomplished using NaOH/methyliodide. MALDI-TOF-MS was performed with an HP LDI 1700XP mass spectrometer operated in the positive ion mode at 30 kV and with a pressure of 6 x 10-7 Torr. The mass spectrometer was calibrated with a mixture of glucose oligomers (degree of polymerization between 3 and 20). Aqueous solutions of samples were diluted 13 with aqueous 50% acetonitrile containing 100 mM 2,5-dihydroxybenzoic acid, and a portion (0.5 (L) was applied to the probe tip of the mass spectrometer. Samples were desorbed from the probe tip with a nitrogen laser ((=337 nm) having a pulse width of 3 ns and delivering approximately 16 (J of energy/laser pulse.

样品被氯化并通过MALDI-MS分析 2,5-二羟基苯甲酸基质。 样品的甲基化为 使用NaOH/甲基碘化物完成。 使用MALDI-TOF-MS进行 HP LDI 1700xp质谱仪以正离子模式运行 在30 kV，压力为6 x 10-7托尔。 质谱仪 用葡萄糖低聚物的混合物进行校准（程度 聚合在3至20之间）。 样品的水溶液是 用含有100毫米的水溶剂水溶液稀释13 2,5-二羟基苯甲酸和一部分（0.5（l）应用于 质谱仪的探针尖端。 样品从 具有氮激光器的探针尖端（（（= 337 nm），脉冲宽度为3 NS并提供约16个（能量/激光脉冲的J。