GLYCOSYL LINKAGE ANALYSIS OF WATER SOLUBLE & INSOLUBLE LINKED GLUCANS

水溶性的糖基连接分析

• 批准号: 6653599
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653599
• 关键词: biological products; biomedical resource; carbohydrates; drug /agent; spectrometry

The sample was permethylated and analyzed by MALDI-MS using a 2,5-dihydroxybenzoic acid matrix. Methylation of the sample was accomplished using NaOH/methyliodide. MALDI-TOF-MS was performed with an HP LDI 1700XP mass spectrometer operated in the positive ion mode at 30 kV and with a pressure of 6 x 10-7 Torr. The mass spectrometer was calibrated with a mixture of glucose oligomers (degree of polymerization between 3 and 20). Aqueous solutions of samples were diluted 13 with aqueous 50% acetonitrile containing 100 mM 2,5-dihydroxybenzoic acid, and a portion (0.5 (L) was applied to the probe tip of the mass spectrometer. Samples were desorbed from the probe tip with a nitrogen laser ((=337 nm) having a pulse width of 3 ns and delivering approximately 16 (J of energy/laser pulse.

将样品全甲基化，并通过MALDI-MS使用 2，5-二羟基苯甲酸基质。 样品的甲基化是 使用NaOH/碘甲烷完成。 MALDI-TOF-MS进行， 在正离子模式下操作的HP LDI 1700 XP质谱仪 在30 kV和6 × 10-7 Torr的压力下。 质谱仪 用葡萄糖低聚物的混合物校准（葡萄糖低聚物的程度）。 聚合度在3和20之间）。 样品的水溶液被 用含有100 mM的50%乙腈水溶液稀释13 5-二羟基苯甲酸，并将一部分（0.5（L））施加到 质谱仪的探针尖端。 样品从 具有氮激光器（=337 nm）的探针尖端，其具有脉冲宽度3 ns，并提供大约16（J的能量/激光脉冲。