GLYCOSYL COMPOSITION ANALYSIS OF COLLAGEN SAMPLES

胶原蛋白样品的糖基成分分析

• 批准号: 6653591
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653591
• 关键词: biological products; biomedical resource; carbohydrates; spectrometry

For the FAB-MS analysis, all experiments were performed on a JEOL SX/SX102A tandem four-sector mass spectrometer operated at 10 kV accelerating potential. Ions were produced by fast atom bombardment with xenon using a JEOL FAB gun operated at 6 kV. The spectra were recorded in the positive ion mode with a resolution of approximately 1000 (90% valley). The spectra are averaged profile data of two scans as recorded by a JEOL complement data system. These spectra were acquired from 100 to 2500 m/z at a rate that would scan from m/z 100 to m/z 2500 in 1 minute with a filtering rate of 300 Hz.

对于FAB-MS分析，所有实验均在JEOL上进行。 SX/SX 102 A串联四扇区质谱计（10 kV） 加速潜力 离子是由快速原子轰击产生的 使用在6 kV下操作的JEOL FAB枪与氙接触。 光谱是 以正离子模式记录，分辨率约为 1000（90%谷值）。 光谱是两次扫描的平均轮廓数据 如由JEOL补码数据系统记录的。 这些光谱是 从100到2500 m/z采集，速率为从m/z 100 以300 Hz的过滤速率在1分钟内分离至m/z 2500。