GLYCOSYL COMPOSITION ANALYSIS OF COLLAGEN SAMPLES

胶原蛋白样品的糖基成分分析

• 批准号: 6653591
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653591
• 关键词: biological products; biomedical resource; carbohydrates; spectrometry

For the FAB-MS analysis, all experiments were performed on a JEOL SX/SX102A tandem four-sector mass spectrometer operated at 10 kV accelerating potential. Ions were produced by fast atom bombardment with xenon using a JEOL FAB gun operated at 6 kV. The spectra were recorded in the positive ion mode with a resolution of approximately 1000 (90% valley). The spectra are averaged profile data of two scans as recorded by a JEOL complement data system. These spectra were acquired from 100 to 2500 m/z at a rate that would scan from m/z 100 to m/z 2500 in 1 minute with a filtering rate of 300 Hz.

对于FAB-MS分析，所有实验都在JEOL上进行 SX/SX102A型串联式10千伏四段质谱仪 加速势。离子是由快原子轰击产生的。 使用JEOL FAB喷枪工作在6千伏的氙气。这些光谱是 以正离子模式记录，分辨率约为 1000(90%山谷)。光谱是两次扫描的平均轮廓数据 如JEOL补充数据系统所记录的。这些光谱是 以100到2500 m/z的速度获取，扫描速度为m/z 100 以300赫兹的滤波率在1分钟内达到m/z 2500。