COURSES IN SEPARATION & CHARACTERIZATION OF COMPLEX CARBOHYDRATES

分离课程

• 批准号: 6653606
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653606
• 关键词: bacteria; biomedical resource; carbohydrates; spectrometry

Glycosyl composition analysis was performed on Ehrlichia risticii lipopolysaccharides. The samples were analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The samples were then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

里氏埃立克氏菌的糖基组成分析 脂多糖。对样品进行了单糖分析。 通过制备三甲基硅基衍生物制备的组合物 甲基葡萄糖苷-气相色谱-结合气相色谱 色质联用(GC-MS)分析。TMS 样品经3M甲醇裂解制得甲基苷 然后用盐酸与吡啶和乙酸酐进行N-乙酰化。这个 然后用Tri-sil处理样品。TMS的气相色谱分析 甲基糖苷在HP5890 GC上使用DB1熔融硅胶进行 毛细管柱。使用HP5890 GC进行GC-MS分析 再配上5970MSD。单糖的鉴定是通过它们的 与标准相比的保留时间，以及碳水化合物 这些化合物的性质通过它们的质谱图进行了鉴定。