COURSES IN SEPARATION & CHARACTERIZATION OF COMPLEX CARBOHYDRATES

分离课程

• 批准号: 6653606
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653606
• 关键词: bacteria; biomedical resource; carbohydrates; spectrometry

Glycosyl composition analysis was performed on Ehrlichia risticii lipopolysaccharides. The samples were analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The samples were then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

对里斯特埃里希体进行糖基组成分析 脂多糖 分析样品中的单糖 通过制备三甲基甲硅烷基的三甲基甲硅烷基衍生物， 甲基糖苷，然后进行气相色谱（GC）和合并的气体 色谱/质谱（GC-MS）分析。 TMS 通过在3 M甲醇中甲醇分解从样品制备甲基糖苷 HCl，然后用吡啶和乙酸酐进行N-乙酰化。 的 然后用Tri-Sil处理样品。 TMS的GC分析 甲基糖苷在HP 5890 GC上使用DB 1熔融二氧化硅 毛细管柱 使用HP 5890 GC进行GC-MS分析 与5970型默沙东手枪相连 单糖通过其 与标准品相比的保留时间，以及碳水化合物 这些化合物的质谱特征得到了证实。