GLYCOSYL COMPOSITION ANALYSIS OF YEAST CELL WALL MATERIAL

酵母细胞壁材料的糖基组成分析

• 批准号: 6653601
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653601
• 关键词: bacteria; biomedical resource; carbohydrates; skin; spectrometry

The sample was analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The samples were then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

通过对样品进行单糖组成分析 甲基糖苷三甲基硅基衍生物的制备 其次是气相色谱(GC)和组合气体 色质联用(GC-MS)分析。TMS 样品经3M甲醇裂解制得甲基苷 然后用盐酸与吡啶和乙酸酐进行N-乙酰化。这个 然后用Tri-sil处理样品。TMS的气相色谱分析 甲基糖苷在HP5890 GC上使用DB1熔融硅胶进行 毛细管柱。使用HP5890 GC进行GC-MS分析 再配上5970MSD。单糖的鉴定是通过它们的 与标准相比的保留时间，以及碳水化合物 这些化合物的性质通过它们的质谱图进行了鉴定。