Selective Breeding for Insulin Growth Factor 1 in Mice

小鼠胰岛素生长因子 1 的选择性育种

• 批准号: 7140392
• 负责人: GERALD E MCCLEARN
• 金额: $ 14.16万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7140392
• 关键词: aging; animal breeding; binding proteins; body composition; body physical activity; body weight; family genetics; generation difference; genetic models; insulinlike growth factor; laboratory mouse; model design /development; phenotype; protein metabolism

DESCRIPTION (provided by applicant): This proposal is for the initial phase of a program to generate a purpose-built animal model system for exploring the relevance of insulin-like growth factor-1 (IGF-1) to aging processes. The data collected during this first phase will address the feasibility of the general strategy, and will provide key information for establishing the design parameters for the subsequent program. The broad strategy is to utilize selective breeding in mice to generate lines diverging extensively in plasma levels of IGF-1. This general approach has proved of immense value in many biological research domains. Success in any particular application, however, depends principally upon the heritability of the trait under investigation. The proposed research will assess IGF-1 levels and candidate correlated traits of IGFBP-3, body weight, total body fat, and physical activity in 120-day-old animals of a parent and an offspring generation of genetically heterogeneous mice. Heritabilities of each phenotype will be estimated by parent-offspring regression, phenotypic correlations among the phenotypes will be assessed, and the degree to which the covariance is due to genetic correlation will be estimated by parent-offspring cross-covariance analyses. Stability of these variables and their interrelationships will be determined by reassessment of the parent generation at 400 days of age.

描述（由申请人提供）：该提案适用于生成专用动物模型系统的计划的初始阶段，该系统旨在探索胰岛素样生长因子-1 (IGF-1) 与衰老过程的相关性。第一阶段收集的数据将解决总体策略的可行性，并将为建立后续计划的设计参数提供关键信息。总体策略是利用小鼠的选择性育种来产生血浆 IGF-1 水平差异很大的品系。这种通用方法已被证明在许多生物学研究领域具有巨大价值。然而，任何特定应用的成功取决于 主要取决于所研究性状的遗传力。拟议的研究将评估 IGF-1 水平和 IGFBP-3 候选相关特征、体重、全身脂肪和身体状况 120天龄动物的父母和具有遗传异质性的后代的活动 老鼠。每个表型的遗传力将通过亲子回归、表型 将评估表型之间的相关性，以及协方差的程度 遗传相关性将通过亲子交叉协方差分析来估计。 这些变量的稳定性及其相互关系将通过在 400 天龄时对亲代进行重新评估来确定。