A Replacement 300 Kv IVEM for Meso-scale 3D Data Acquisition at the NCMIR

描述（由申请人提供）：该提案要求提供资金，以支持购买现代化的高性能中压透射电子显微镜（IVEM），以取代老化的日本电子光学（JEOL）JEM-4000 EX，该仪器已作为厚切片自动3D成像（电子断层扫描，电子光学切片）的生产仪器近17年。该仪器将取代位于圣地亚哥加州大学校园内的国家显微镜和成像研究中心（NCIR）的JEM-4000 EX。NCMIR是NCRR/NIH生物医学技术研究中心，成立于1989年，旨在开发计算机辅助先进显微镜，用于采集1 nm 3至100 um 3尺寸范围内的结构和功能数据，该尺寸范围被称为“meso”或中间尺度。该仪器的主要功能是能够持续支持越来越多的驱动应用项目，这些项目需要在细胞和生物组织厚的1至3 μ m切片的中尺度3D成像领域的定制性能和功能。

DESCRIPTION (provided by applicant): This proposal requests funds to support the acquisition of a modern, high performance intermediate voltage transmission electron microscope (IVEM) to replace an aging Japanese Electron Optics (JEOL) JEM- 4000EX that has served as a production instrument for automated 3D imaging of thick sections (electron tomography, electron optical sectioning) for nearly 17 years. This instrument will physically supplant the JEM-4000EX at the National Center for Microscopy and Imaging Research (NCMIR) on the campus of the University of California, San Diego. The NCMIR is an NCRR/NIH Biomedical Technology Research Center established in 1989 to develop computer-aided advanced microscopy for acquisition of structural and functional data in the dimensional range of 1 nm3 to 100 um3, a dimensional range referred to as the "meso" or middle scales. This instrument will serve the primary function of enabling the continued support of an increasing base of driving application projects that require tailored performance and functionality in the area of meso-scale, 3D imaging of thick, 1 to 3 um sections of cells and biological tissues.