A Replacement 300 Kv IVEM for Meso-scale 3D Data Acquisition at the NCMIR

描述（由申请人提供）：本提案要求资金支持购买现代高性能中压透射电子显微镜（IVEM），以取代老化的日本电子光学（JEOL） JEM- 4000EX，该JEM- 4000EX已作为厚切片（电子断层扫描，电子光学切片）的自动3D成像生产仪器近17年。该仪器将取代位于加州大学圣地亚哥分校的国家显微镜与成像研究中心（NCMIR）的JEM-4000EX。NCMIR是NCRR/NIH生物医学技术研究中心，成立于1989年，旨在开发计算机辅助高级显微镜，用于获取尺寸范围为1nm3至100um3的结构和功能数据，该尺寸范围被称为“中尺度”或中等尺度。该仪器的主要功能是为不断增长的驱动应用项目提供持续支持，这些项目需要在1至3微米厚的细胞和生物组织切片的中尺度3D成像领域定制性能和功能。

DESCRIPTION (provided by applicant): This proposal requests funds to support the acquisition of a modern, high performance intermediate voltage transmission electron microscope (IVEM) to replace an aging Japanese Electron Optics (JEOL) JEM- 4000EX that has served as a production instrument for automated 3D imaging of thick sections (electron tomography, electron optical sectioning) for nearly 17 years. This instrument will physically supplant the JEM-4000EX at the National Center for Microscopy and Imaging Research (NCMIR) on the campus of the University of California, San Diego. The NCMIR is an NCRR/NIH Biomedical Technology Research Center established in 1989 to develop computer-aided advanced microscopy for acquisition of structural and functional data in the dimensional range of 1 nm3 to 100 um3, a dimensional range referred to as the "meso" or middle scales. This instrument will serve the primary function of enabling the continued support of an increasing base of driving application projects that require tailored performance and functionality in the area of meso-scale, 3D imaging of thick, 1 to 3 um sections of cells and biological tissues.