A Replacement 300 Kv IVEM for Meso-scale 3D Data Acquisition at the NCMIR

描述(由申请人提供)：本建议书申请资金支持购买一台现代化的高性能中压传输电子显微镜(IVEM)，以取代老化的日本电子光学(JEOL)JEM-4000EX，JEM-4000EX近17年来一直是厚切片(电子断层扫描、电子光学切片)自动化3D成像的生产仪器。这台仪器将在加州大学圣地亚哥分校的国家显微镜和成像研究中心(NCMIR)实际取代JEM-4000EX。NCMIR是美国国立卫生研究院/美国国立卫生研究院在成立的生物医学技术研究中心，目的是开发计算机辅助高级显微镜，以获取1微米3到100微米3的尺寸范围内的结构和功能数据，该尺寸范围被称为“中”尺度。该仪器的主要功能是能够继续支持越来越多的驱动应用项目，这些项目需要在1至3微米厚的细胞和生物组织切片的中尺度3D成像方面具有量身定制的性能和功能。

DESCRIPTION (provided by applicant): This proposal requests funds to support the acquisition of a modern, high performance intermediate voltage transmission electron microscope (IVEM) to replace an aging Japanese Electron Optics (JEOL) JEM- 4000EX that has served as a production instrument for automated 3D imaging of thick sections (electron tomography, electron optical sectioning) for nearly 17 years. This instrument will physically supplant the JEM-4000EX at the National Center for Microscopy and Imaging Research (NCMIR) on the campus of the University of California, San Diego. The NCMIR is an NCRR/NIH Biomedical Technology Research Center established in 1989 to develop computer-aided advanced microscopy for acquisition of structural and functional data in the dimensional range of 1 nm3 to 100 um3, a dimensional range referred to as the "meso" or middle scales. This instrument will serve the primary function of enabling the continued support of an increasing base of driving application projects that require tailored performance and functionality in the area of meso-scale, 3D imaging of thick, 1 to 3 um sections of cells and biological tissues.