MESA Family - Ocular Component

MESA家族研究旨在应用最先进的基因分型和基因分析方法，通过研究参与NHLBI赞助的动脉粥样硬化多种族研究(MESA-Parent)的一组个人的兄弟姐妹和选定的其他家庭成员，来描绘动脉粥样硬化和其他心血管疾病亚临床措施的遗传基础。梅萨家庭研究由NHLBI资助，首席研究员是锡达斯-西奈医学中心的杰罗姆·罗特。除了六个现场中心外，这项研究还包括一个协调中心、一个中心实验室、两个基因分析中心和计算机断层扫描、超声和视网膜摄影阅读中心。评估视力和视网膜病理的眼科方案与父母MESA研究中使用的方案相同，MESA家族也采用相同的眼科方案。 MESA家族的主要目标包括确定450名拉美裔美国人和450名非洲裔美国人的兄弟姐妹并进行表型分析；在这900个兄弟姐妹中进行10 cM的全基因组搜索和连锁分析，以确定可能包含导致心血管(和眼睛)表型差异的基因的区域；以及对至少3,000个MESA指数病例的数据进行遗传关联分析，以调查(或测试)先前确定的候选基因，并评估候选基因在参与母公司MESA研究的不同种族群体中的作用。 梅萨家庭兄弟姐妹的招募工作于2004年5月开始，预计将于2006年秋季完成。此后，将开始对梅萨家族成员进行遗传分析。2006年初完成了3,000个MESA指数病例的初步基因分型；数据清理活动和初步分析正在进行中。

The MESA Family study is designed to apply state-of-the-art genotyping and genetic analysis methodologies to delineate the genetic basis underlying atherosclerosis and other subclinical measures of cardiovascular disease by studying the siblings and selected other family members of a subset of individuals who participated in the NHLBI-sponsored Multi-Ethnic Study of Atherosclerosis (MESA-Parent). The MESA Family study is funded as a grant by NHLBI and the Principal Investigator is Jerome Rotter at Cedars-Sinai Medical Center. In addition to six field centers, the study involves a Coordinating Center, a Central Laboratory, two Genetic Analysis Center, and Reading Centers for Computed Tomography, Ultrasound and Retinal Photography. The same ocular protocols for assessing vision and retinal pathology as those used in the parent MESA Study are employed for MESA Family. The main objectives for the MESA Family include the ascertainment and phenotyping of 450 Hispanic American and 450 African American sibships; a 10cM genome-wide search and linkage analysis in these 900 sibships to identify regions likely to contain genes contributing to variation in the cardiovascular (and ocular) phenotypes; and the conduct of genetic association analyses on data from at least 3,000 MESA index cases to investigate (or test previously identified) candidate genes and to evaluate their role of the candidate genes within the different ethnic groups participating in the parent MESA study. Recruitment of MESA Family sibships began in May 2004 and is expected to be completed in Fall 2006. Genetic analyses for the MESA Family component will commence thereafter. Initial genotyping of the 3,000 MESA index cases was completed early 2006; data cleaning activities and preliminary analyses are underway.