STUDIES OF RUBREDOXIN BY PARAMAGNETIC 15N NMR SPECTROSCOPY

顺磁 15N 核磁共振波谱法研究红红氧还蛋白

• 批准号: 7420549
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 1.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-20 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420549
• 关键词: fluid; hydrogen bond; micelles; mutant; reduction; temperature

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Several site-mutagenesis on hydrogen bonds of Cp rubredoxin have been studied and reduction potentials of these mutants are reported recently. Paramagnetic NMR is powerful to examine the H bonds associated with reduction potential of Cp rebredoxin. In addition, rubredoxin in reverse micelles in liquid propane provides the wider temperature-dependent study of hyperfine shifts.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。近年来，人们对Cp rubredoxin的氢键进行了定点突变研究，并报道了这些突变体的还原电位。顺磁NMR是强大的检查与Cp rebredoxin的还原电位相关的H键。此外，rubredoxin在反胶束中的液体丙烷提供了更广泛的温度依赖性的超精细位移的研究。