Host genes controlling Flavivirus infection: new insights and application for developing highly effective Kunjin replicon-based Ebola vaccine

控制黄病毒感染的宿主基因：开发高效昆金复制子埃博拉疫苗的新见解和应用

• 批准号: nhmrc : GNT1102421
• 负责人: Prof Alexander Khromykh
• 金额: $ 73.7万
• 依托单位国家: 澳大利亚
• 项目类别: Project Grants
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 无数据
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/host-genes-controlling-ebola-vaccine/1346214
• 关键词: Host; genes; controlling; Flavivirus; infection

The applications is aimed at identifying new host genes controlling infection with West Nile virus and other medically important flaviviruses such as dengue and Japanese encephalitis. For this, we will use novel in vivo RNAi screening approach with virus libraries encoding artificial microRNAs (amirs) targeting whole mouse genome. We will then apply amiR technology to produce highly effective Kujniin replicon-based Ebola vaccine candidate that has shown promising results in trails in primates.

这些应用旨在鉴定控制西尼罗河病毒和其他医学上重要的黄病毒如登革热和日本脑炎感染的新宿主基因。为此，我们将使用新的体内RNAi筛选方法，使用编码靶向整个小鼠基因组的人工microRNA（amirs）的病毒文库。然后，我们将应用amiR技术生产高效的基于Kujniin复制子的埃博拉候选疫苗，该疫苗在灵长类动物试验中显示出有希望的结果。