METHODS FOR PRODUCTION OF ICSI AND SCNT DERIVED MACAQUE STEM CELLS

ICSI 和 SCNT 衍生的猕猴干细胞的生产方法

• 批准号: 7957964
• 负责人: GERALD SCHATTEN
• 金额: $ 24.7万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-06 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957964
• 关键词: Animal Model; Autistic Disorder; Computer Retrieval of Information on Scientific Projects Database; Data; Derivation procedure; Diabetes Mellitus; Disease; Ejaculation; Embryo; Embryo Cloning; Fertilization; Freezing; Funding; Future; Germ Cells; Goals; Grant; Heart Diseases; Immune; Institution; Intracytoplasmic Sperm Injections; Laboratories; Laparotomy; Macaca; Methods; Modeling; Normalcy; Oocytes; Parkinson Disease; Pharmaceutical Preparations; Pilot Projects; Primates; Production; Protocols documentation; Research; Research Personnel; Resources; Safety; Source; Staging; Stem cells; Testing; Therapeutic; United States National Institutes of Health; Work; blastocyst; embryo culture; embryonic stem cell; established cell line; human embryonic stem cell; nonhuman primate; pluripotency; rectal; somatic cell nuclear transfer; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this protocol, macaques will be hormonally stimulated with super-ovulatory drugs to produce oocytes that will be collected via laparotomy. These oocytes will be fertilized in the laboratory using fresh or frozen sperm collected by rectal probe electro-ejaculation. The collected gametes will be used for fertilization in the laboratory by intracytoplasmic sperm injection and the embryos cultured to the blastocyst stage. Once reaching this stage, several methods will be employed to attempt to derive embryonic stem cells. In addition, some oocytes may undergo somatic cell nuclear transfer to form cloned embryos. These embryos will also be cultured to the blastocyst stage in order to derive stem cells. Long term, we anticipate that this research will enable us to optimize methods for the derivation of macaque embryonic stem cells from both fertilized and SCNT embryos. However, for this pilot project, our goal is to simply obtain data on cyno follicular stimulation and determine whether or not cynos may be an ideal animal model for future work. Any established cell lines will undergo rigorous testing to determine their pluripotency and normalcy. While human embryonic stem cells may have incredible therapeutic value for treating a wide range of disorders such as Parkinson's diabetes, autism, heart disease, Alzheimer's etc., rigorous testing in animal models is necessary before any such trials can begin. Macaques provide a valuable non-human primate model in which to determine the safety and efficacy of immune-matched stem cells.

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