IN-SITU UV/VIS SPECTROSCOPY FOR MACROMOLECULAR CRYSTALLOGRAPHY

用于大分子晶体学的原位紫外/可见光谱

• 批准号: 7721935
• 负责人: S M SOLTIS
• 金额: $ 1.15万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721935
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Crystallography; Data; Electronics; Funding; Goals; Grant; In Situ; Institution; Measurement; Metals; Oxidation-Reduction; Proteins; Research; Research Personnel; Resources; Source; Spectrum Analysis; System; United States National Institutes of Health; absorption; base; instrument; oxidation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to enable the simultaneous acquisition of x-ray diffraction data and single crystal absorption spectra in the UV/Visible and infrared regions. Analysis of the absorption spectrum in the UV (190 - 400 nm) and visible (400 - 800 nm) regions provides information about the electronic state of proteins while in the crystalline state. To explore this approach, a single crystal microspectrophotometer was set up on BL7-1 to record spectra from crystals on the goniometer. During diffraction experiments, spectroscopic measurements were analyzed to confirm the redox state. Data were collected from crystals containing mixtures of metal oxidation states. The resulting data allow the estimation of the mixed states. Based on these and previous experiments, the instrument is now being automated and incorporated into the beam line control system.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的目标是能够同时采集紫外/可见光和红外区域的 X 射线衍射数据和单晶吸收光谱。 对紫外 (190 - 400 nm) 和可见光 (400 - 800 nm) 区域的吸收光谱的分析提供了有关蛋白质在结晶状态下的电子状态的信息。 为了探索这种方法，在 BL7-1 上安装了单晶显微分光光度计来记录测角仪上晶体的光谱。 在衍射实验期间，分析光谱测量以确认氧化还原状态。 从含有金属氧化态混合物的晶体中收集数据。 所得数据可以估计混合状态。 基于这些和之前的实验，该仪器现在正在实现自动化并并入光束线控制系统中。