IN-SITU UV/VIS SPECTROSCOPY FOR MACROMOLECULAR CRYSTALLOGRAPHY

用于大分子晶体学的原位紫外/可见光谱

• 批准号: 7721935
• 负责人: S M SOLTIS
• 金额: $ 1.15万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721935
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Crystallography; Data; Electronics; Funding; Goals; Grant; In Situ; Institution; Measurement; Metals; Oxidation-Reduction; Proteins; Research; Research Personnel; Resources; Source; Spectrum Analysis; System; United States National Institutes of Health; absorption; base; instrument; oxidation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to enable the simultaneous acquisition of x-ray diffraction data and single crystal absorption spectra in the UV/Visible and infrared regions. Analysis of the absorption spectrum in the UV (190 - 400 nm) and visible (400 - 800 nm) regions provides information about the electronic state of proteins while in the crystalline state. To explore this approach, a single crystal microspectrophotometer was set up on BL7-1 to record spectra from crystals on the goniometer. During diffraction experiments, spectroscopic measurements were analyzed to confirm the redox state. Data were collected from crystals containing mixtures of metal oxidation states. The resulting data allow the estimation of the mixed states. Based on these and previous experiments, the instrument is now being automated and incorporated into the beam line control system.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的目标是能够同时获得紫外/可见光和红外区的X射线衍射数据和单晶吸收光谱。对紫外(190-400 nm)和可见光(400-800 nm)区域的吸收光谱的分析提供了有关蛋白质在结晶态时的电子状态的信息。为了探索这一方法，在BL7-1上安装了一台单晶显微分光光度计，在测角仪上记录晶体的光谱。在衍射实验中，对光谱测量进行了分析，以确认氧化还原状态。数据是从含有金属氧化态混合物的晶体中收集的。由此得到的数据允许对混合状态的估计。基于这些和以前的实验，该仪器现在正被自动化，并被整合到束线控制系统中。