Core--Multiphoton imaging /electrophysiology

多光子成像/电生理核心的目的是为分析蛋白质定位、蛋白质动力学和蛋白质-蛋白质相互作用提供高分辨率的仪器。该设备还将允许用户对活细胞和组织中的多个荧光团进行定时成像，并将荧光标记蛋白质或离子指示染料的高分辨率成像与电活动的电生理监测相结合。该核心由蔡司LSM 510 META NLO共聚焦显微镜锚定，配有两个可见光激光器用于执行传统的单光子共聚焦成像，一个近红外脉冲激光器用于双光子成像。此外，这台显微镜的舞台有能力要么是封闭的，以创建一个长期延时成像的培养箱，或被电生理设备包围，以允许同时全细胞记录和成像。该设施的独特功能将为JHU SOM的其他NINDS资助的研究人员提供资源，并将建立研究神经生物学过程的新方法，这将使JHU社区受益。该核心的设备和能力在JHU的任何核心设施中都没有复制。

The aim of the Multiphoton Imaging/EIectrophysiology Core is to provide instrumentation for analyzing protein localization, protein dynamics, and protein-protein interactions with high resolution. This facility will also allow users to perform time-lapse imaging of multiple fluorophores in living cells and tissues, and combine high resolution imaging of fluorescently tagged proteins or ion indicator dyes with electrophysiological monitoring of electrical activity. This Core is anchored by a Zeiss LSM 510 META NLO confocal microscope equipped with two visible lasers for performing traditional single-photon confocal imaging, and one near-infrared pulsed laser for two-photon imaging. In addition, the stage of this microscope has the capacity to either be enclosed to create an incubator for long-term time-lapse imaging, or to be surrounded by electrophysiology equipment to allow simultaneous whole-cell recording and imaging. The unique capabilities of this facility will serve as a resource for other NINDS funded investigators at JHU SOM and will establish new approaches for studying neurobiological processes that will benefit the greater JHU community. The equipment and capabilities of this core are not duplicated in any core facilities at JHU.