RECOVERY OF EPIDIDYMAL SPERM FROM RHESUS MACAQUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 背景：为了确定附睾精子能否被取回并成功冷冻保存，在尸检时采集了20只恒河猴的睾丸。方法：男性，年龄3~19岁，平均8.5岁。3只4岁以下的动物均未获得精液。其余17个样本含有精液，平均精子密度为144.0毫微克/毫升，平均活动率为72.8%，平均等级为2.6%。结果/讨论：培养24小时后，所有样本的总活力均显着下降，24小时时新鲜未激活精液和激活精液的总活力分别为8.8%和3.8%。冷冻对未激活精液的平均评分为1.6，解冻后即刻活率为42.4%，冷冻效果显著。在新鲜精液中，激活4小时后精子活力进一步降低(72.8vs50.4%)，而在冷冻精液中则增加(14.0vs23.2%)。男性的年龄不影响精液的浓度、级别或初始活力，但在决定冷冻解冻激活精液的活力时，从老年男性样本中发现活力较低的显著因素。总体而言，这项研究表明，活动精液可以从死后男性身上获得，尽管后续的研究将不得不确定其质量是否足以促进其在辅助生殖中的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: To determine whether epididymal sperm could be retrieved and successfully cryopreserved testes were collected at necropsy from a total of 20 rhesus macaques. Methods: The males ranged in age from 3 to 19 years with an average of 8.5 years. No semen was obtained from three animals, which were younger than 4 years. The remaining 17 samples contained semen with an average sperm cell concentration of 144.0 mio/mL, an average motility of 72.8 % and a mean grade of 2.6. Results/Discussion: After 24 h of culture the overall motility decreased significantly in all samples regardless of treatment to 8.8 % in fresh non-activated and 3.8 % in activated semen at 24 hr. Freezing had a significant effect on semen reducing the mean grade to 1.6 and the immediate post-thaw motility to 42.4 % in non-activated samples. Activation further reduced semen motility after 4 hr in fresh semen (72.8 vs 50.4%), but increased it in semen that had been frozen (14.0 vs 23.2%). The age of the male did not influence semen concentration, grade or initial motility, but proved to be a significant factor in determining motility of frozen-thawed activated semen with lower motility found in samples from older males. Overall the study demonstrates that motile semen can be obtained from post-mortem males, although subsequent studies will have to determine whether the quality is sufficient to facilitate its use in assisted reproduction.