GENETICS AND GENOME BANKING CORE

遗传学和基因组库核心

• 批准号: 8173011
• 负责人: Hans Michael Kubisch
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173011
• 关键词: Alleles; Animals; Back; Blood; Blood Banks; Blood specimen; Cell Line; Chinese People; Computer Retrieval of Information on Scientific Projects Database; DNA; Equilibrium; Equipment and supply inventories; Fibroblasts; Funding; Gene Frequency; Generations; Genetic; Genome; Genotype; Grant; Institution; Methods; Microsatellite Repeats; Output; Parents; Partner in relationship; Research; Research Personnel; Resources; Source; Tandem Repeat Sequences; United States National Institutes of Health; base; father role; genetic resource; male; offspring

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: The efforts of the Core have focused on banking of genetic resources and on generation of microsatellites to assign paternity and determine allele frequencies. Methods: Blood was collected during routine inventories from which we have amplified small tandem repeats (STRs). The PCR products were sent to UC Davis for size determination. The sequencer output was sent back to us and Core staff makes the final size interpretations of each allele and asigns genotypes. Results/Discussion: During the past year we received 594 STR profiles from animals born from 1983 through 2007 that were based on an older STR panel of 13 loci. From these we have calculated allele frequencies and have determined that the level of heterozygosity has not changed when animals born before and after 2000 are compared. We have established STR profiles using the expanded panel of 34 loci from 230 animals (an additional 190 animals await fragment size determinations at UC Davis), which included 216 Indian and 14 Chinese males. Analysis of these STRs similarly showed a high degree of heterozygosity that does not deviate significantly from what would be expected under equilibrium conditions, such as completely random mating. (observed heterozygosity: 0.75, expected heterozygosity: 0.77). Among the 216 Indian animals were 22 offspring, 109 potential dams and 44 potential sires. We have established paternity for 11 and maternity for 4 of these animals. We do not yet have STR profiles on file for all parents and final parentage determinations will be done when those have arrived. We have collected blood samples from 817 animals, and have obtained DNA extracts from 362 animals. This brings the number of blood-banked animals to 4,356.A total of 100 fibroblast cell lines have been established and cryopreserved, with an additional 50 lines currently in culture.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 简介：核心小组的工作重点是建立遗传资源库，并制作微卫星，以确定亲子关系和等位基因频率。 方法：在常规库存期间收集血液，我们从中扩增了小串联重复序列（STR）。将PCR产物送至UC Davis进行大小测定。测序仪的输出被送回给我们，核心工作人员对每个等位基因的大小进行最终解释，并指定基因型。 结果/讨论：在过去的一年里，我们收到了594份来自1983年至2007年出生的动物的STR图谱，这些图谱是基于13个基因座的旧STR面板。根据这些数据，我们计算了等位基因频率，并确定了在2000年前后出生的动物进行比较时，杂合性水平没有变化。我们使用来自230只动物（另外190只动物在加州大学戴维斯分校等待片段大小测定）的34个基因座的扩展组建立了STR图谱，其中包括216只印度雄性和14只中国雄性。 对这些STR的分析同样显示了高度的杂合性，其不显著偏离在平衡条件下（例如完全随机交配）的预期。（观察杂合性：0.75，预期杂合性：0.77）。 在216只印度动物中，有22只后代，109只潜在的母畜和44只潜在的公畜。我们已经确定了其中11只动物的父亲和4只动物的母亲。我们还没有所有父母的STR档案，最终的亲子关系确定将在这些档案到达后进行。 我们收集了817只动物的血液样本，并从362只动物中提取了DNA。这使得血库中的动物数量达到4,356只。总共建立并冷冻保存了100个成纤维细胞系，目前还有50个细胞系正在培养中。