GENETIC TRANSFORMATION OF MOUSE EMBRYOS

小鼠胚胎的遗传转化

• 批准号: 8172947
• 负责人: Hans Michael Kubisch
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172947
• 关键词: Animals; Area; Computer Retrieval of Information on Scientific Projects Database; Embryo; Female; Funding; Genetic Transformation; Grant; Housing; Human Chorionic Gonadotropin; Individual; Injection of therapeutic agent; Institution; Litter Size; Location; Longevity; Maintenance; Methods; Mus; Oocytes; Ovum; Production; Research; Research Personnel; Resources; Source; Superovulation; System; Testing; Transgenic Mice; United States National Institutes of Health; design; male

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: While static and ventilated caging systems are both widely utilized in transgenic mouse production facilities, the effects of caging on mouse oocyte and progeny production have not been sufficiently analyzed. This study was designed to compare the oocyte yield of FVB and B6 mice on individual ventilated and static isolated cages. Methods: FVB and B6 mice were maintained in either static or ventilated cages. Females were subjected to superovulation by injections of FHS and hCG and placed with males. Ova were collected 44 hrs later. Results/Discussion: There appeared to be no differences in oocyte yield between FVB and B6 females or caging system. The average oocyte per female in the static vs. ventilated caging system was 18 vs. 21 in FVB and 31 vs. 31 in B6, respectively. We subsequently tested the females' oocyte yield when they were housed in different areas of the ventilated rack (VR). Animals in cages located at the bottom o the VR produced more oocytes than those animals placed at the middle or top of the rack. To further determine whether this location effect could influence the progeny yield, FVB and ICR dams - six groups per strain at two mice per cage, were paired with one male each. The breeder trios were housed at the middle or the bottom of VR, and their litters and litter size were tracked for one year. Dams housed at the bottom of the VR produced significantly more litters than those housed at the middle of the rack, which was mostly due to a longer life span. This information should prove useful for colony management for both transgenic mouse production and general breeder colony maintenance.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景资料：虽然静态和通风笼系统都被广泛用于转基因小鼠生产设施，笼小鼠卵母细胞和后代生产的影响还没有得到充分的分析。本研究旨在比较FVB和B6小鼠在单独通风和静态隔离笼中的卵母细胞产量。 方法：将FVB和B6小鼠饲养在静态或通风笼中。通过注射FHS和hCG对雌性进行超数排卵，并与雄性放置在一起。44小时后收集卵。 结果/讨论：FVB和B6雌性动物或笼养系统之间的卵母细胞产量似乎无差异。 静态与通风笼养系统中每只雌性动物的平均卵母细胞分别为18 vs. 21（FVB）和31 vs. 31（B6）。随后，我们测试了雌性的卵母细胞产量，当它们被安置在通风架（VR）的不同区域时。 位于VR底部的笼子中的动物比放置在架子中间或顶部的动物产生更多的卵母细胞。 为了进一步确定这种位置效应是否会影响后代产量，将FVB和ICR母鼠（每个品系六组，每个笼子两只小鼠）与各一只雄性配对。 饲养员三人组被安置在VR的中间或底部，并跟踪他们的窝仔和窝仔数一年。 安置在VR底部的母鼠比安置在机架中间的母鼠产下的窝仔明显更多，这主要是由于寿命更长。 这一信息应证明是有用的转基因小鼠生产和一般育种群体的维护的群体管理。