GENETICS AND GENOME BANKING CORE

遗传学和基因组库核心

• 批准号: 7958696
• 负责人: Hans Michael Kubisch
• 金额: $ 5.8万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958696
• 关键词: Animals; Blood specimen; Breeding; Cell Line; Collection; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Library; Data; Deformity; Equipment and supply inventories; Fibroblasts; Funding; Generations; Genome; Grant; Individual; Infant; Institution; Methods; Microsatellite Repeats; Paper; Parents; Phenotype; Primates; Research; Research Personnel; Resources; Sampling; Source; Tremor; United States National Institutes of Health; Work; base; cranium; father role; genetic resource; offspring; working group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: The primary objective of the core is on banking of genetic resources and on generation of microsatellites to assign paternity. Methods: To date we have predominantly concentrated our efforts on the collection of blood samples from animals that are taken during inventory of the breeding corrals. We have collected blood samples from nearly 3520 animals and have blotted these samples onto archival filter paper. We have also extracted DNA from 116 of the collected samples. We have established primary fibroblast cell lines from 6 animals. These have included two homozygous Krabbe infants (HJ76, HR85) as well as two animals with unusual phenotypes (EA32, skull deformity; EC39, tremors) and their dams. The NPRC Genome Banking Working Group decided to establish DNA banks of representative animals at each of the primate centers consisting of 50 unrelated individuals and ten trios each containing both parents and one offspring. Results/Discussion: To date we have collected blood samples from 30 unrelated individual animals and from nine trios. We have generated microsatellites based on a set of markers developed by David Glen Smith at UC Davis. This set consists of 34 satellites arranged into 6 individual panels. To date we have generated satellites from 116 animals and have sent these to Dr. Smith's lab for fragment size analysis. We expect to obtain electropherogram data shortly and will use these to assign paternity. Work on the next group of animals is ongoing.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 导言：该核心的主要目标是建立遗传资源库和生成微卫星以确定亲子关系。研究方法：到目前为止，我们主要集中精力从育种库库存期间采集的动物中采集血样。我们收集了近3520只动物的血液样本，并将这些样本涂抹在存档滤纸上。我们还从收集的116个样本中提取了DNA。我们已经建立了6只动物的原代成纤维细胞系。其中包括两个纯合子Krabbe婴儿（HJ 76，HR 85）以及两个具有异常表型的动物（EA 32，颅骨畸形; EC 39，震颤）和它们的母体。 NPRC基因组库工作组决定在每个灵长类中心建立代表性动物的DNA库，包括50个无关个体和10个三人组，每个三人组包含父母和一个后代。结果/讨论：到目前为止，我们已经从30只无关的个体动物和9只三胞胎动物中采集了血样。我们已经根据加州大学戴维斯分校的大卫格伦史密斯开发的一组标记生成了微卫星。这套卫星由34颗卫星组成，排列成6个独立的面板。 到目前为止，我们已经从116只动物身上产生了卫星，并将它们送到史密斯博士的实验室进行碎片大小分析。我们希望很快获得电泳图谱数据，并将使用这些数据来确定亲子关系。下一组动物的工作正在进行中。