GENETICS AND GENOME BANKING CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 导言：该中心的主要目标是建立遗传资源库和开发微卫星来分配亲子关系。方法：到目前为止，我们主要集中精力收集动物的血液样本，这些样本是在对繁殖畜栏进行清查期间采集的。我们收集了近3520只动物的血液样本，并将这些样本涂抹在档案滤纸上。我们还从收集的116个样本中提取了DNA。我们建立了6种动物的原代成纤维细胞系。其中包括两个纯合子Krabbe婴儿(HJ76，HR85)以及两个具有不寻常表型的动物(EA32，头骨畸形；EC39，震颤)及其母马。NPRC基因组库工作组决定在每个灵长类中心建立具有代表性的动物的DNA库，每个中心由50个无关的个体和10个三元组组成，每个三元组包含父母和一个后代。结果/讨论：到目前为止，我们已经从30只没有血缘关系的个体动物和9个三人组采集了血液样本。我们已经根据加州大学戴维斯分校的大卫·格伦·史密斯开发的一套标记生成了微型卫星。这组卫星由34颗卫星组成，排列成6个独立的面板。到目前为止，我们已经从116只动物身上制造了卫星，并将这些卫星送到史密斯博士的实验室进行碎片大小分析。我们预计很快就会获得电泳图数据，并将用这些数据来确定亲子关系。关于下一组动物的工作正在进行中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: The primary objective of the core is on banking of genetic resources and on generation of microsatellites to assign paternity. Methods: To date we have predominantly concentrated our efforts on the collection of blood samples from animals that are taken during inventory of the breeding corrals. We have collected blood samples from nearly 3520 animals and have blotted these samples onto archival filter paper. We have also extracted DNA from 116 of the collected samples. We have established primary fibroblast cell lines from 6 animals. These have included two homozygous Krabbe infants (HJ76, HR85) as well as two animals with unusual phenotypes (EA32, skull deformity; EC39, tremors) and their dams. The NPRC Genome Banking Working Group decided to establish DNA banks of representative animals at each of the primate centers consisting of 50 unrelated individuals and ten trios each containing both parents and one offspring. Results/Discussion: To date we have collected blood samples from 30 unrelated individual animals and from nine trios. We have generated microsatellites based on a set of markers developed by David Glen Smith at UC Davis. This set consists of 34 satellites arranged into 6 individual panels. To date we have generated satellites from 116 animals and have sent these to Dr. Smith's lab for fragment size analysis. We expect to obtain electropherogram data shortly and will use these to assign paternity. Work on the next group of animals is ongoing.